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Mutafchiev Yasen Y Department of Animal Diversity and Resources, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, - - 2014
In the present study, we describe intraperitoneal development of the FR3 strain of Brugia malayi in Mongolian jirds (Meriones unguiculatus). The third molt for male worms occurred between 4 and 7 days postinfection (dpi) and between 4 and 8 dpi for females. The fourth and final molt occurred between days 21 ...
Relevancy Score: Relevancy Score: 590
Subrahmanyam D - - 1990
Monoclonal antibodies (mAbs) have been prepared against excretory-secretory-metabolic (ESM) antigens of microfilariae (mf) of Wuchereria bancrofti (WbmfESM) and against third stage larvae (L3) of Brugia malayi (BmL3), and purified from ascites fluids with ammonium sulphate. Both antibodies were of the IgM type and did not react with phosphorycholine. The mAb ...
Relevancy Score: Relevancy Score: 589
Barbee W C - - 1977
Domestic cats were infected with third-stage Brugia malayi in such a way that the parasites were restricted to the regional lymphatics of one hind limb. Later, these cats were exposed on the same leg to the yeast phase of Sporothrix schenckii. Edema and fibrosis were more extensive in cats infected ...
Relevancy Score: Relevancy Score: 589
Bosworth W - - 1977
This study evaluated the usefulness of determining antistreptolysin O(ASO) titers in filariasis. The ASO titers were elevated in most convalescence sera, but did not reflect comparable pathology resulting from dual Brugia-streptococcal infections in cats.
Relevancy Score: Relevancy Score: 588
Michalski Michelle L - - 2002
Brugia malayi is a filarial nematode parasite that causes lymphatic filariasis, a disease that affects millions of people in the tropics. Sexual reproduction of filarial worms occurs within the lymphatic vessels of the human host and is crucial for transmission of the parasite to the mosquito vector. We have previously ...
Relevancy Score: Relevancy Score: 588
O'Holohan D R - - 1974
Relevancy Score: Relevancy Score: 588
Foster Jeremy - - 2005
Complete genome DNA sequence and analysis is presented for Wolbachia, the obligate alpha-proteobacterial endosymbiont required for fertility and survival of the human filarial parasitic nematode Brugia malayi. Although, quantitatively, the genome is even more degraded than those of closely related Rickettsia species, Wolbachia has retained more intact metabolic pathways. The ...
Relevancy Score: Relevancy Score: 587
Lal R B - - 1987
Filarial parasites induce immune response in humans which are still poorly characterized. To define the antigens responsible for inducing lymphocyte responses, fast protein liquid chromatography was used to fractionate the antigens of Brugia malayi adult worms which were then tested on lymphocytes from patients with filariasis and normal controls. From ...
Relevancy Score: Relevancy Score: 587
Lal R B - - 1990
The immunosuppressive effect of Brugia malayi antigen (BmA) on phytohemagglutinin (PHA) driven T cell proliferation was evaluated in patients with filariasis (n = 14) and compared to control individuals (n = 12). When peripheral blood lymphocytes were co-cultured with BmA and PHA, BmA markedly suppressed the T cell proliferative response ...
Relevancy Score: Relevancy Score: 587
Hiil J L - - 1988
Mass drug administration via 3 modes of delivery reduced the incidence and prevalence rates and intensity of Brugia malayi infection in 3 rural villages in the Bengkoka Peninsula, Sabah, in 1982-1983. A dosage of 6 mg diethylcarbamazine citrate (DEC-C)/kg body weight was administered either daily or weekly (total of 6 ...
Relevancy Score: Relevancy Score: 587
Aggarwal A - - 1985
Several monoclonal antibodies were produced following the immunization of mice with infective larvae of Brugia malayi. One of these gives a positive fluorescence reaction on the surface of B. malayi microfilariae and this particular monoclonal antibody (IgM isotype) was able to mediate mouse peritoneal macrophage adherence to, and killing of, ...
Relevancy Score: Relevancy Score: 584
Spencer H C - - 1981
Sequentially collected sera from Mongolian jirds (Meriones unguiculatus) infected with Brugia malayi and B. pahangi were tested for antibodies to homologous and heterologous antigens by the enzyme-linked immunosorbent assay (ELISA). Titers were less than 1:100 prior to infection and rose rapidly (within 2 weeks). Peak titers were observed prior to ...
Relevancy Score: Relevancy Score: 584
Li B W - - 1999
Immunization with recombinant Brugia malayi paramyosin protein (BM5) induces partial immunity to this filarial nematode in jirds. The present study examined the effects of intramuscular immunization with plasmid DNA that encodes BM5. DNA-immunized mice produced strong antibody and cell-mediated responses to paramyosin. The protective activity of DNA vaccination with BM5 ...
Relevancy Score: Relevancy Score: 584
Raverdy Sylvine - - 2008
Genome analysis of the glycolytic/gluconeogenic pathway in the Wolbachia endosymbiont from the filarial parasite Brugia malayi (wBm) has revealed that wBm lacks pyruvate kinase (PK) and may instead utilize the enzyme pyruvate phosphate dikinase (PPDK; ATP:pyruvate, orthophosphate phosphotransferase, EC 2.7.9.1). PPDK catalyses the reversible conversion of AMP, PPi and phosphoenolpyruvate ...
Relevancy Score: Relevancy Score: 584
Jamail M - - 2005
We conducted a field study of a rapid test (Brugia Rapid) for detection of Brugia malayi infection to validate its sensitivity and specificity under operational conditions. Seven districts in the state of Sarawak, Malaysia, which are endemic for brugian filariasis, were used to determine the test sensitivity. Determination of specificity ...
Relevancy Score: Relevancy Score: 582
Allen J E - - 1996
Specific T cell hyporesponsiveness and depressed antibody production is a key feature of human infection with the filarial nematodes, Brugia malayi and Wuchereria bancrofti. Despite this immune suppression, responses indicative of Th2 subset activation are present, including unusually high levels of specific IgG4. We tested the possibility that infection with ...
Relevancy Score: Relevancy Score: 582
Dixit S - - 2004
The study was aimed at identifying pro- and anti-inflammatory cytokine releasing potential of Brugia malayi adult worm fractions and their role in filarial infection and pathogenesis. THP-1 cells were incubated with soluble somatic Brugia malayi adult worm extract (BmAS) and its Sephadex G-200 fractions BmAFI, BmAFII and BmAFIII and the ...
Relevancy Score: Relevancy Score: 582
Pearlman E - - 1993
The role of specific Th subsets in the regulation of acquired resistance to the filarial parasite Brugia malayi is not known. We examined pathologic and cytokine responses in filarial antigen-sensitized BALB/c mice inoculated intraperitoneally with live microfilariae. Animals immunized three times with soluble microfilarial antigen demonstrated accelerated clearance of live ...
Relevancy Score: Relevancy Score: 582
Sabesan S - - 1991
Rates of acquisition and loss of Brugia malayi microfilaraemia were estimated using the parasitological data of a cohort of population in Shertallai, South India. The rate of acquisition of microfilaraemia was found to be dependent on age but not gender. The decline in the rate of acquisition of microfilaraemia in ...
Relevancy Score: Relevancy Score: 582
Pfarr K M - - 2001
The radical gas nitric oxide (NO) is synthesized by nitric oxide synthase (NOS) from l-arginine and molecular oxygen. Nitric oxide is an important signaling molecule in invertebrate and vertebrate systems. Previously we have shown that NOS is localized to more tissues in Brugia malayi than has been reported in Ascaris ...
Relevancy Score: Relevancy Score: 581
Smith V P - - 1998
We have examined the susceptibility of cuticular membrane lipids of Brugia malayi to oxidants generated in vitro. Live parasites as well as extracted cuticular lipids were treated with hydrogen peroxide and hypochlorous acid and the extent of lipid peroxidation was quantified. The cuticular membranes of B. malayi were found to ...
Relevancy Score: Relevancy Score: 581
Wu Bo - - 2009
Filarial parasites (e.g., Brugia malayi, Onchocerca volvulus, and Wuchereria bancrofti) are causative agents of lymphatic filariasis and onchocerciasis, which are among the most disabling of neglected tropical diseases. There is an urgent need to develop macro-filaricidal drugs, as current anti-filarial chemotherapy (e.g., diethylcarbamazine [DEC], ivermectin and albendazole) can interrupt transmission ...
Relevancy Score: Relevancy Score: 581
Krushna Nagampalli S A - - 2009
cDNA coding for Brugia malayi pepsin inhibitor homolog (Bm-33) from the human filarial parasite was cloned in pRSET for large-scale expression and functional characterization. The pRSET-B cloned gene did not yield recombinant protein expression and the reason was attributed to the presence of an N-terminal signal peptide. The gene was ...
Relevancy Score: Relevancy Score: 581
Gnanasekar Munirathinam - - 2008
This is the first report of a tetraspanin (TSP)-like molecule in the lymphatic filarial parasites. Expressed sequence tag (EST) database search for TSP like molecules in the filarial genome resulted in three significant EST hits (two partial ESTs from Brugia malayi and one full length EST from Wuchereria bancrofti). The ...
Relevancy Score: Relevancy Score: 580
Lengemann F W - - 1986
Ten radioisotopes (133Ba, 207Bi, 109Cd, 51Cr, 59Fe, 203Hg, 54Mn, 125Sb and 75Se) were used to label the L-3 stage of Brugia malayi. The in vitro uptake at 4 hours in a buffered salt solution was greatest for 207Bi and exceeded that of 203Hg, by a factor of 6 and the ...
Relevancy Score: Relevancy Score: 579
Sim B K - - 1982
Serum dependent cell-mediated cytotoxicity of subperiodic Brugia malayi infective larvae in vitro was investigated. In vitro cellular adherence of normal human buffy coat cells to infective larvae of B. malayi was promoted by sera from patients with elephantiasis, tropical pulmonary eosinophilia (TPE) and amicrofilaraemic symptomatic filariasis, as well as by ...
Relevancy Score: Relevancy Score: 579
Kumar N P - - 1998
Laboratory studies conducted on Ma. annulifera collected from Cherthala region, Kerala, endemic for Brugian filariasis indicated that this species is highly susceptible to B. malayi parasites. The experimental infection index of this species was relatively higher (2.74). No significant loss of larvae during development was recorded. The extrinsic incubation period ...
Relevancy Score: Relevancy Score: 578
Ibrahim M S - - 1992
Surface and metabolic labeling procedures were used to characterize the composition and the time of expression of Brugia malayi L2 and L3 surface-associated molecules as the larvae develop within the mosquito vector. Larvae were harvested from mosquito tissues at 5 (early L2), 8 (late L2) and 11 (L3) days post-infection ...
Relevancy Score: Relevancy Score: 578
Fan P C - - 1985
Infective larvae of subperiodic B. malayi from South Kalimantan (Borneo), Indonesia collected from laboratory-raised Ae. togoi mosquitoes after feeding on infected mongolian gerbils (Meriones unguiculatus) were inoculated subcutaneously into the groin areas of 15 SD and 36 LE rats. Blood was examined weekly by membrane filtration and thick smears starting ...
Relevancy Score: Relevancy Score: 578
Pearlman E - - 1995
The purpose of the current study was to evaluate the effects of exogenous IL-12 on: 1) development of the Th cell response to Brugia malayi microfilariae (mf); 2) established B. malayi-specific Th2 responses; and 3) resistance to mf. IL-12 given at the time of inoculation with live mf resulted in ...
Relevancy Score: Relevancy Score: 577
Murthy P K - - 2008
The influence of live Brugia malayi parasites and a Sephadex G-200 fraction of the adult parasite extract (BmAFII) on the progression of Leishmania donovani infection was studied. Inbred hamsters were first infected with B. malayi infective 3rd stage larvae (L3), adult worms or microfilariae (mf), and then with L. donovani ...
Relevancy Score: Relevancy Score: 577
Gupta R - - 2004
The present report compares the macrophage function in rodent hosts susceptible and resistant to the human lymphatic filariid Brugia malayi. Macrophages from both mastomys (resistant) and gerbil (susceptible) infected intraperitoneally (i.p.) with the infective larvae (L3) of B. malayi were isolated from peritoneal lavage at different time-intervals and formation rate ...
Relevancy Score: Relevancy Score: 577
Triteeraprapab S - - 2001
Lymphatic filariasis caused by Brugia malayi is highly prevalent in Narathiwat province of Thailand. The World Health Organization has aimed to eliminate the disease globally by the year 2020. To achieve the goal, assessment of the real disease situation should be integrated as part of the control program. The preliminary ...
Relevancy Score: Relevancy Score: 577
Falcone F H - - 1995
The in vitro cultivation of the filarial nematode Brugia malayi from the infective stage to the fourth and the young adult stage is described. Different culture conditions including cell-free systems and co-culture with different human lymphatic cell lines were compared. Cell-free systems reported by others to promote the in vitro ...
Relevancy Score: Relevancy Score: 577
Li B W - - 1993
Vaccination with irradiated infective larvae induces partial protective immunity to infection with the filarial nematode Brugia malayi in jirds. Prior studies have shown that such immunization stimulates a much stronger antibody response to recombinant and native filarial paramyosin than that seen after normal infection. To determine whether vaccination with recombinant ...
Relevancy Score: Relevancy Score: 577
Ardelli B F - - 2010
ABC systems are one of the largest described protein superfamilies. These systems have a domain organization that may contain 1 or more transmembrane domains (ABC_TM1F) and 1 or 2 ATP-binding domains (ABC_2). The functions (e.g., import, export and DNA repair) of these proteins distinguish the 3 classes of ABC systems. ...
Relevancy Score: Relevancy Score: 577
Page A P - - 1995
The cyclophilins are a family of proteins that exhibit peptidyl-prolyl cis-trans isomerase (PPIase, EC 5.2.1.8) activity and bind the immunosuppressive agent cyclosporin A (CsA) to varying degrees. We have isolated a cDNA clone encoding a novel cyclophilin from the human filarial parasite Brugia malayi. This gene possesses an N-terminal domain ...
Relevancy Score: Relevancy Score: 577
Srivastava Y - - 2004
A 175 kDa antigen fraction with collagenase activity was isolated and purified from somatic extracts of adult Setaria cervi females using column chromatography involving consecutive steps of DEAE-Sepharose CL6B and Sephadex G-100. The optimum pH for 175 kDa collagenase was found to be pH 7.0. Sensitivities to a variety of ...
Relevancy Score: Relevancy Score: 577
Shu Limin - - 2003
Biolistic transient transfection of Brugia malayi embryos with constructs driving the expression of a luciferase reporter gene was used to identify regions of the upstream sequence of the heat shock protein 70 (HSP70) gene of B. malayi necessary for transgene expression. Analysis of 1160 nucleotides upstream of the start codon ...
Relevancy Score: Relevancy Score: 577
Dabir P - - 2006
The Brugia malayi filarial antigens recognised preferentially by sera from an endemic normal population are considered to be potential vaccine candidates. By immunoscreening the cDNA library of the infective L3 stage of B. malayi with pooled endemic normal sera, a cDNA clone Bm-SL3 was identified. Analysis of sera from different ...
Relevancy Score: Relevancy Score: 577
Simons Jonathan E - - 2005
Using two models of filarial infection in which Brugia malayi microfilariae (Mf) are contained in distinct anatomical compartments, in blood or tissue sites, we have demonstrated a critical role for eotaxin-1 in parasite clearance. In the first model, implantation of adult B. malayi into the peritoneal cavity of eotaxin-1(-/-) mice ...
Relevancy Score: Relevancy Score: 577
Dabir Snehal - - 2008
The immunoscreening of Brugia malayi adult cDNA library with pooled endemic normal sera identified several seroreactive clones including, EC-SOD which contained a 612 bp insert and showed significant nucleotide and deduced amino acid sequence homologies with superoxide dismutase (SOD) of other nematode parasites. The SODs are known to play an ...
Relevancy Score: Relevancy Score: 577
McSorley Henry J HJ Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, United - - 2008
Many helminths, including Brugia malayi, are able to establish long-lived infections in immunocompetent hosts. Growing evidence suggests that the immune system's failure to eliminate parasites is at least partially due to the effects of regulatory T cells (Tregs). To test whether parasites may directly stimulate host regulatory activity, we infected ...
Relevancy Score: Relevancy Score: 577
Ewert A - - 1983
This experimental study showed that daily oral administration of DEC at levels of 5 to 15 mg/kg/day with food for 3 weeks decreased the level of both developing and adult B. malayi in infected cats. There were no adverse reactions due to the medication. Topical application of 5% DEC in ...
Relevancy Score: Relevancy Score: 577
Maizels R M - - 1983
Surface antigens of three stages of three species of the filarial nematode genus Brugia have been analysed by radio-iodination and immunoprecipitation. These surface antigens have been shown to be characteristic for each stage by polyacrylamide gel electrophoresis. For example, infective larvae and adult worms have relatively complex patterns while microfilariae ...
Relevancy Score: Relevancy Score: 576
Lu Y R - - 1991
Under the experimental conditions of 26 +/- 10 degrees C, relative humidity 70-80%, 100 +/- 20 Lux and photoperiod 16 hours/day and using a membrane feeding method, the development of filarial larvae and the variations in reproductive capacity, gonotrophic cycle and longevity of An. sinensis infected with microfilariae of Brugia ...
Relevancy Score: Relevancy Score: 576
Araujo A - - 1994
Ultrastructural analysis of the cuticle of Brugia malayi microfilariae indicated that it is composed of 2 regions: the inner one 15-20 nm thick with a homogeneous aspect and the outer one, designated as epicuticle, which is 15-20 nm thick. Three laminae separated by electron-lucent regions were seen in the epicuticle. ...
Relevancy Score: Relevancy Score: 576
Falcone Franco H. - - 1995
The in vitro cultivation of the filarial nematode Brugia malayi from the infective stage to the fourth and the young adult stage is described. Different culture conditions including cell-free systems and coculture with different human lymphatic cell lines were compared. Cocultivation with each of the cell lines used enhanced the ...
Relevancy Score: Relevancy Score: 576
Aboobaker A Aziz - - 2003
We describe the successful use of the reverse genetic technique RNA interference (RNAi) to investigate gene function in the human filarial nematode parasite Brugia malayi. We used fluorescently labelled double stranded RNA (dsRNA) to demonstrate that 300 bp molecules are able to enter adult females in culture while they remain ...
Relevancy Score: Relevancy Score: 576
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