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Gengin M T - - 1995
It was established that the activity of carboxypeptidase H increases in divisions of the brain of rats during a one-time emotional-algic stress. The most substantial increase in the activity of the enzyme is observed in the hypophysis and the hypothalamus. The changes in activity were less marked with chronic emotional-algic ...
Relevancy Score: Relevancy Score: 483
Paslen V V - - 1981
Semi-empirical calculation of conformational properties of covalent complexes of carboxypeptidase A with ester and peptide substrates are presented. There is a favourable form of transient tetrahedral and covalent acyl-enzyme intermediates in the active site of carboxypeptidase A. The ester and peptide substrates bind in the active site of carbopeptidase A ...
Relevancy Score: Relevancy Score: 483
Hilsted L - - 1986
Using fragment 5-17 of human gastrin-17 extended with glycine at the C-terminus as hapten, three of six rabbits produced high-titer, high-avidity antisera specific for glycine-extended gastrins. In combination with trypsin and carboxypeptidase B cleavage, radioimmunoassays based on these antisera measured progastrins in some extra-antral tissues and certain malignant tumors. The ...
Relevancy Score: Relevancy Score: 483
Gengin M T - - 1995
The activity of carboxypeptidase H involved in metabolism of neuropeptides was studied in rats resistant and predisposed to emotional stress under normal and stress conditions. The enzymatic activity was higher in the striatum and hypothalamus of the rats predisposed to stress than that of stress-resistant animals. Emotional stress resulted in ...
Relevancy Score: Relevancy Score: 483
Eun H M - - 1978
A DD-carboxypeptidase activity is present in membrane fragments of Mycobacterium smegmatis. Kinetic parameters of the enzymatic activity have been studied using UDP-N-glycolylmuramyl-L-alanyl-gamma-D-glutamyl-meso-2,2'-diaminopimelyl-D-[14C]alanyl-D-[14C]alanine as substrate. The DD-carboxypeptidase can be solubilized by Triton X-100 and Genapol X-100. It is inhibited by beta-lactam antibiotics although intact cells of M. smegmatis are insensitive to ...
Relevancy Score: Relevancy Score: 483
Nagamori Y - - 1990
An intracellular protease from a bacterium, Bacillus pumilus HL721, was purified about 5000-fold by chromatography with a Q-Sepharose Fast Flow column, TSK-gel HA-1000 glass column, and TSK-gel G3000SWXL column using Bz-Gly-Ala-Pro as a substrate. The enzyme was the most active at pH around 7.5 and stable from 4.5 and 8.0. ...
Relevancy Score: Relevancy Score: 483
Nagamori Y - - 1991
Enzymatic properties of dipeptidyl carboxypeptidase (DCP) from Bacillus pumilus were investigated. The enzyme was more active on tri- and tetrapeptides than angiotensin-converting enzyme (ACE) from rabbit lung. The presence of chloride ion is essential for the hydrolysis. The Km value of angiotensin I for the enzyme was 0.119 x 10(-3) ...
Relevancy Score: Relevancy Score: 483
Cisneros R J - - 1993
The binding of 5-fluorodeoxyuridylate (FdUMP) to carboxypeptidase-inactivated thymidylate synthase obtained from methotrexate-resistant Lactobacillus casei was investigated using [3H]FdUMP in a trichloroacetic acid precipitation assay and by 19F nuclear magnetic resonance spectroscopy. The cleavage of 1 valine residue from the carboxyl terminus of one of the identical subunits of the enzyme ...
Relevancy Score: Relevancy Score: 482
Galardy R E - - 1985
The aldehyde (RS)-2-benzyl-4-oxobutanoic acid, which is 25% hydrated at pH 7.5, has recently been shown to be a strong reversible competitive inhibitor of carboxypeptidase A [Ki = 0.48 nM; Galardy, R. E., & Kortylewicz, Z. P. (1984) Biochemistry 23, 2083-2087]. The ketone analogue of this aldehyde (RS)-2-benzyl-4-oxopentanoic acid (IV) is ...
Relevancy Score: Relevancy Score: 482
Schilf W - - 1980
Two membrane-bound enzymes of Proteus mirabilis with the dual functions of peptidoglycan DD-carboxypeptidase and transpeptidase (named DD-carboxypeptidase/transpeptidase H and L) were isolated and purified by selective solubilization with the nonionic detergent Genapol X-100, affinity chromatography on matrix-bound ampicillin, and preparative isoelectric focusing in the presence of detergent. Purified enzymes H ...
Relevancy Score: Relevancy Score: 482
Castaño L - - 1991
Using serum from a prediabetic patient as a probe, we screened 0.5 x 10(6) recombinants from a rat islet lambda gt11 expression library. One plaque-producing antigen reactive with this prediabetic serum was identified, subcloned, and sequenced. Analysis of the sequence reveals that the clone encodes a 136-amino acid fragment of ...
Relevancy Score: Relevancy Score: 482
Fuad N - - 1976
The exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39 is inhibited by beta-lactam antibiotics according to the same general scheme of reaction as the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61. However, the values for the kinetic constants involved in the reaction are very different for the two enzymes and provide an explanation for the ...
Relevancy Score: Relevancy Score: 482
Wang Lai - - 2009
Carboxypeptidase Z (CPZ) removes carboxyl-terminal basic amino acid residues, particularly arginine residues, from proteins. CPZ contains a cysteine-rich domain (CRD) similar to the CRD found in the frizzled family of Wnt receptors. We have previously shown that thyroid hormone regulates terminal differentiation of growth plate chondrocytes through activation of Wnt-4 ...
Relevancy Score: Relevancy Score: 482
Rasmussen J R - - 1978
The penicillin-sensitive D-alanine carboxypeptidases of Bacillus subtilis, Escherichia coli, and Staphylococcus aureus catalyzed the hydrolysis of the D-lactic acid residue from the depsipeptide diacetyl-L-lysyl-D-alanyl-D-lactic acid. The ester substrate was hydrolyzed faster than the peptide analogue, diacetyl-L-lysyl-D-alanyl-D-alanine, by the B. subtilis (15-fold) and E. coli (4-fold) carboxypeptidases, presumably because acylation (k(2)), ...
Relevancy Score: Relevancy Score: 482
Mangani S - - 1992
The structure of the carboxypeptidase A complex with the inhibitor (S)-(+)-1-amino-2-phenylethylphosphonic acid has been determined at 0.23 nm resolution. The delta F map shows electron-density peaks both in the S1 and S'1 sites, where the inhibitor molecule can be modeled in two different orientations with approximate 50% occupancy. In the ...
Relevancy Score: Relevancy Score: 482
Dua R D - - 1984
The carboxypeptidase A purified from goat pancreas has been found to have a molecular weight of 34,600 +/- 300. The enzyme is a zinc-protein and the molar ratio of zinc to enzyme protein is 1:1. Removal of zinc yields an inactive apocarboxypeptidase A. The loss of activity of the native ...
Relevancy Score: Relevancy Score: 482
Hook V Y - - 1988
1. Carboxypeptidase H is one of several proteolytic processing enzymes required for conversion of large neuropeptide precursors into the small peptide neurotransmitters and hormones. 2. Because of the importance of posttranslational processing as a regulatory step for the production of active peptides, recent studies investigating control mechanisms for carboxypeptidase H ...
Relevancy Score: Relevancy Score: 482
Moews P C - - 1981
The sequence homology found by Waxman & Strominger between penicillin-sensitive D-alanine-carboxypeptidases and penicillin-inactivating beta-lactamases is shown to extend to the level of secondary structure as predicted by the method of Chou & Fasman or by the informational method of Garnier et al. Thermodynamic similarity of homologous segments of these proteins ...
Relevancy Score: Relevancy Score: 482
Igić Rajko - - 2003
In this study we demonstrate that carboxypeptidase A (CPA)-like enzyme is expressed in rat kidney. The major metabolites of angiotensin (Ang) I by the rat renal mesangial cell extract at 37 degrees C, pH 7.4, were Ang 1-9 and Ang II. Quinaprilat did not influence the formation of Ang 1-9, ...
Relevancy Score: Relevancy Score: 481
Muszynska G - - 1976
Nitration of bovine carboxypeptidase A crystals with tetranitromethane increases esterase activity, decreases peptidase activity, and modifies about one tyrosyl residue. Modification of enzyme crystals avoids the polymerization that occurs when the enzyme is nitrated in solution. Two procedures have been employed to identify the tyrosyl residues nitrated. The first involves ...
Relevancy Score: Relevancy Score: 481
Breslow R - - 1978
The enzyme carboxypeptidase A has been extensively studied and is thus a good candiate for chemical models and imitation. A model system was constructec which combined two of the three catalytic functional groups of the enzyme together with the appropriate substrate group, and cooperative effective catalysis was demonstrated. However, the ...
Relevancy Score: Relevancy Score: 481
Narahashi Y - - 1990
The amino acid sequence of a zinc-carboxypeptidase from S. griseus (Cpase SG) was determined by automated Edman degradation and carboxypeptidase digestion of the S-carboxymethylated protein and by sequence analyses of peptides produced by cyanogen bromide cleavage and by lysyl endopeptidase digestion of the S-carboxymethylated protein. This enzyme is characterized by ...
Relevancy Score: Relevancy Score: 481
Pearce G - - 1984
Carboxypeptidase inhibitor from potato tubers was fed to newly hatched chicks at a level equal to that present in diet containing 50% raw potato, which caused severe growth depression and 20% mortality. At this level the effects of the inhibitor on growth was small but the following effects were noted: ...
Relevancy Score: Relevancy Score: 481
Takahashi Y - - 1975
Previously an enzyme, named acylagmatine amidohydrolase, hydrolyzing bleomycin B2 to bleomycinic acid and agmatine was found in the mycelia of Fusarium anguioides Sherbakoff. In this work the enzyme was purified further, but not completely. The crude enzyme preparation hydrolyzed various acylagmatines and also peptidyl arginine, but the latter activity could ...
Relevancy Score: Relevancy Score: 481
Ribeiro J M - - 1998
Saliva and salivary gland homogenates of Ixodes scapularis contain a dipeptidyl carboxypeptidase activity that accounts for the previously described salivary kininase activity of this tick. Reversed phase HPLC and laser desorption mass spectrography of the reaction products identified bradykinin fragment 1-7 and 1-5 as being produced subsequent to incubation of ...
Relevancy Score: Relevancy Score: 481
Ferrer Marc - - 2003
An epitope-unmasking, homogeneous time-resolved fluorescence (HTRF) assay has been developed for measuring carboxypeptidase B (CPB) activity in a miniaturized high-throughput screening format. The enzyme substrate (biotin-RYRGLMVGGVVR-OH) is cleaved by CPB at the C terminus, causing release of the C-terminal Arg residue. The product (biotin-RYRGLMVGGVV-OH) is recognized specifically by a monoclonal ...
Relevancy Score: Relevancy Score: 481
Rees D C - - 1980
The structure of the complex between the proteolytic enzyme carboxypeptidase A (peptidyl-L-amino-acid hydrolase, EC 3.4.17.1) and the 39-amino-acid carboxypeptidase A inhibitor from potatoes has been determined at 2.5-A resolution. A combination of multiple isomorphous replacement, molecular replacement, and noncrystallographic symmetry averaging techniques was used to solve the structure. The chain ...
Relevancy Score: Relevancy Score: 480
Galardy R E - - 1984
DL-2-Benzyl-3- formylpropanoic acid ( XIVb ) is a competitive inhibitor of carboxypeptidase A with an apparent Ki of 0.48 microM at pH 7.5 in 50 mM Tris buffer-0.5 M in sodium chloride with O-(trans-p- chlorocinnamoyl )-L-beta-phenyllactate as substrate. At pH 7.5 in deuterium oxide, DL-2-benzyl-3- formylpropanoic acid exists as an ...
Relevancy Score: Relevancy Score: 480
Fricker L D - - 1983
Enkephalin convertase, an enkephalin-synthesizing carboxypeptidase present in adrenal medulla chromaffin granules, has also been detected in brain and pituitary. To determine whether these three carboxypeptidase activities represent the same enzyme, we purified and characterized enkephalin convertase from adrenal medulla, whole brain, and whole pituitary. Enzyme from all three tissues co-purifies ...
Relevancy Score: Relevancy Score: 480
Mock W L - - 2000
Hydroxamic acids of structure RCON(OH)CH(2)CH(CH(2)C(6)H(5))CO(2)H induce micromolar competitive inhibition of catalysis for the enzyme carboxypeptidase A. Enzyme affinity depends on the nature of the acyl group, for RCO equaling HCO, CH(3)CO, FCH(2)CO, F(2)CHCO, F(3)CCO, CH(3)OCH(2)CO, or CH(3)OCO. In acid dissociation these residues yield hydroxamic acid pK(a) values that vary from ...
Relevancy Score: Relevancy Score: 480
Adler Marc - - 2008
This article reports the crystal structures of inhibitors of the functional form of thrombin-activatable fibrinolysis inhibitor (TAFIa). In vivo experiments indicate that selective inhibitors of TAFIa would be useful in the treatment of heart attacks. Since TAFIa rapidly degrades in solution, the homologous protein porcine pancreatic carboxypeptidase B (pp-CpB) was ...
Relevancy Score: Relevancy Score: 480
Roth W W - - 1991
Most peptide hormones are synthesized as part of larger precursor proteins which must be processed after translation to generate bioactive peptides. This usually involves cleavage of the precursor by an endopeptidase at sites marked by basic amino acids, followed by removal of N- or C-terminal basic residues by the action ...
Relevancy Score: Relevancy Score: 480
Auld D S - - 1992
1H NMR spectroscopy of the isotropically shifted signals in cobalt carboxypeptidase, CoCPD, permits a direct and selective detection of protons belonging to the residues liganded to the metal. The chemical shift of these protons in the free enzyme and enzyme-inhibitor complexes with changing pH monitors the state of ionization of ...
Relevancy Score: Relevancy Score: 480
Du Xiao-Yan - - 2009
Chemerin is a potent chemoattractant for cells expressing the serpentine receptor CMKLR1 (chemokine-like receptor 1), such as plasmacytoid dendritic cells and tissue macrophages. The bioactivity of chemerin is post-translationally regulated; the attractant circulates in blood in a relatively inactive form (prochemerin) and is activated by carboxyl-terminal proteolytic cleavage. We discovered ...
Relevancy Score: Relevancy Score: 480
Covaleda Giovanni - - 2012
NvCI is a novel exogenous proteinaceous inhibitor of metallocarboxypeptidases from the marine snail Nerita versicolor. The complex between human carboxypeptidase A4 and NvCI has been crystallized and determined at 1.7 Å resolution. The NvCI structure defines a distinctive protein fold basically composed of a two-stranded antiparallel β-sheet connected by three ...
Relevancy Score: Relevancy Score: 480
Schroeder R L - - 1978
Distal half-kernels of barley (Hordeum vulgare L.), after imbibition for 1 day, produced a carboxypeptidase that was active on N-carbobenzoxy-l-phenylalanyl-l-alanine and on N-carbobenzoxy-l-phenylalanyl-l-phenylalanine. For the ensuing 2 days, activity increased linearly and thereafter increased at reduced rates. Electrofocusing of an imbibed half-kernel homogenate produced coincident peaks of activity on both ...
Relevancy Score: Relevancy Score: 479
Kato T - - 2000
Carboxypeptidase R (EC 3.4.17.20) (CPR) and carboxypeptidase N (EC 3.4.17.3) (CPN) cleave carboxy-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Although CPN is present in a stable active form in plasma, CPR is generated from proCPR, a ...
Relevancy Score: Relevancy Score: 479
Mehta R A - - 1996
Wounding of tomato (Lycopersicon esculentum L.) leaves causes systemic induction of a serine-type carboxypeptidase activity. We find this activity to be present in several isoforms. Antibodies raised against the leaf carboxypeptidase inhibited the enzyme activity and the immunoprecipitates were resolved into a 69-kDa polypeptide and a doublet of 35/37-kDa proteins ...
Relevancy Score: Relevancy Score: 479
Ohno S - - 1980
The effects of carboxypeptidase digestion on the function of colcin E3 which had been demonstrated to be a complex of proteins A and B was investigated. It was indicated that removal of 7 amino acids from the C-terminal region of protein A has no significant effect on the interaction with ...
Relevancy Score: Relevancy Score: 479
Breddam K - - 1991
The applicability of serine carboxypeptidase catalysed transpeptidation reactions, using amino acid amides as nucleophiles, for C-terminal amidation of peptides has been investigated. With the aim of converting an unamidated precursor into GRF(1-29)-NH2, an interesting biologically active derivative of growth hormone releasing factor, a number of model reactions were initially investigated. ...
Relevancy Score: Relevancy Score: 479
Buchanan C E - - 1977
The fact that antibody to d-alanine carboxypeptidase of Bacillus subtilis does not cross-react with other penicillin-binding proteins suggests that these proteins are not precursors or multimers of the enzyme.
Relevancy Score: Relevancy Score: 479
Hadkar Vaishali - - 2004
Membrane-bound regulatory carboxypeptidases cleave only COOH-terminal basic residues from peptides and proteins. To investigate whether carboxypeptidase-generated arginine can increase nitric oxide (NO) synthesis we perfused rat lungs from animals challenged with LPS or used rat lung microvascular endothelial cells (RLMVEC) stimulated with LPS and IFN-gamma, conditions that induced inducible NO ...
Relevancy Score: Relevancy Score: 478
DiSanto M E - - 1992
A developmentally regulated carboxypeptidase was purified from hyphae of the dimorphic fungus Mucor racemosus. The enzyme, designated carboxypeptidase 3 (CP3), has been purified greater than 900-fold to homogeneity and characterized. The carboxypeptidase migrated as a single electrophoretic band in isoelectric focusing polyacrylamide gel electrophoresis (PAGE), with an isoelectric point of ...
Relevancy Score: Relevancy Score: 478
Rabanal R - - 1992
The immunocytochemical detection of amylase, carboxypeptidase A, alpha 1-antitrypsin, carcinoembryonic antigen (CEA) and keratin in normal canine pancreatic tissue and in carcinomas of the exocrine pancreas of the dog is described. In the normal pancreas, the acinar cells contain amylase, carboxypeptidase and alpha 1-antitrypsin. The pancreatic ducts react with the ...
Relevancy Score: Relevancy Score: 478
Ellis P D - - 1989
The liquid-state 113Cd NMR data of carboxypeptidase A in the presence and absence of inhibitors obtained by Gettins (Gettins, P. (1986) J. Biol. Chem. 261, 15513-15518) are analyzed in terms of whether the inhibitors displace water from Cd2+ upon binding to the protein. This question is addressed by applying the ...
Relevancy Score: Relevancy Score: 478
Rybák M - - 1978
Inflammation was induced by application of kaolin into the hind paws of rats and its development under the influence of carboxypeptidase N and aprotinin (Antilysin) was followed and compared with the effect of the phenylbutazone type of drugs. Partly purified rat serum carboxypeptidase N, applied subaponeurally (20 mg/kg) together with ...
Relevancy Score: Relevancy Score: 478
Waniek Peter J PJ Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, FIOCRUZ/RJ, Av Brasil 4365, CEP 21045-900, Rio de Janeiro, RJ, Brazil; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, FIOCRUZ/RJ, Av Brasil 4365, CEP 21045-900, Rio de Janeiro, RJ, Brazil. Electronic address: - - 2014
Using specific oligonucleotides, 5'- and 3'-RACE and sequencing, two cDNAs encoding serine carboxypeptidases (tbscp-1 and tbscp-2) from the midgut of the blood sucking heteropteran Triatoma brasiliensis were identified. Both cDNAs with an open reading frame of 1389 bp, encode serine carboxypeptidase precursors of 463 amino acid residues, which possess a ...
Relevancy Score: Relevancy Score: 478
Ramos C - - 1994
Deletions have been constructed in the region encoding the 91-amino acid propeptide of the vacuolar enzyme carboxypeptidase Y of Saccharomyces cerevisiae, and in vivo effects of these mutations on the intracellular transport of the mutant proenzymes have been examined. Deletions did not include the vacuolar targeting signal, and none of ...
Relevancy Score: Relevancy Score: 478
Dmochowska A - - 1987
The yeast KEX1 gene product has homology to yeast carboxypeptidase Y. A mutant replacing serine at the putative active site of the KEX1 protein abolished activity in vivo. A probable site of processing by the KEX1 product is the C-terminus of the alpha-subunit of killer toxin, where toxin is followed ...
Relevancy Score: Relevancy Score: 478
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