Document Detail
The use of the monocarboxylates β-hydroxybutyrate and lactate in anaerobic exercise and recovery metabolism in the juvenile Pacific spiny dogfish (Squalus acanthias)
Abstract/OtherAbstract :
The Pacific spiny dogfish (Squalus acanthias) is one species of elasmobranch which has demonstrated increased reliance on monocarboxylates as metabolic fuels. This study examined the use of the monocarboxylates β-hydroxybutyrate (β-HB, a ketone body) and lactate (La-) in juvenile Pacific spiny dogfish during exhaustive exercise and recovery using in-vivo tracer analysis. Dogfish in the exercise treatment were hand-chased for 10 minutes until exhaustion followed by 4 hours of recovery while non-exercised dogfish were left at rest for the duration of the experimental period. Scintillation counting and metabolite assays were used to determine metabolite turnover rates, metabolic clearance rate and uptake rate into specific tissues. Exhaustive exercise was found to have no significant effect on any of the parameters measured in the juvenile dogfish. Within the exercise treatment, cardiac concentration and uptake rate of β-HB were found to be significantly higher than that found in muscle and liver tissues. This corresponds with the higher energetic need in highly oxidative cardiac tissues, suggesting that β-HB is an important metabolic fuel here. In the non-exercise treatment lactate levels are significantly higher in the muscle than in other tissues, however not so in the exercise treatment. These results are contrary to previous studies and suggest the dogfish may have not have been under stress previous to experimentation. It is suggested that monocarboxylates play a role in exercise and recovery metabolism of the juvenile spiny dogfish; however more research is required to elucidate their overall importance.
Authors :
Kopf, Victoria
Contributors :
Dr. Yuxiang Wang
Publication Detail :
Publisher :  -     Type :  Thesis     Format :  351328 bytes, application/pdf    
Date Detail :
2008-04-28, 2008-04-28, 2008-04-28
Subject :
beta-hydroxybutyrate, lactate, dogfish, monocarboxylate, exercise
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Other Details :
Languages :  en    
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