| The effects of aB-crystallin on mitochondrial death pathway during hydrogen peroxide induced apoptosis | |
Abstract/OtherAbstract
:
|
aB-crystallin, a major small heat shock protein, has recently been shown to exert inhibitory effects on apoptosis, while the responsible mechanisms remain largely unknown. In the present study, we discovered that aB-crystallin protected mouse myoblast C2C12 cells against oxidative stress-induced apoptosis. During hydrogen peroxide-induced apoptosis, aBcrystallin showed that it decreased the redistribution level of phosphatidylserine (PS), reduced the release of cytochrome C and Smac/Diablo from mitochondria into cytoplasm, and decreased the cleavage of Bid. Interestingly, immunoprecipitation experiments with anti-aBcrystallin and anti-myc-tag antibodies demonstrated respectively an interaction between aBcrystallin and p53 during hydrogen peroxide induced apoptosis. Both the NH2-terminal and COOH-terminal regions of aB-crystallin could interact with p53, suggesting two domains of aB-crystallin are necessary for the interaction. Electrophoresis mobility shift assay (EMSA) and luciferase assay further demonstrated that aB-crystallin inhibited the upregulation of the DNA-binding, as well as the transactivation activity of p53 induced by hydrogen peroxide. Our results show that aB-crystallin has a protective role in oxidative stress induced apoptosis by interference with the mitochondrial death pathway. |
Authors
:
|
Liu, Shuang, Deng, Gonghua, Tu, Zizhi, Li, Junli, Liu, Meidong, Xiao, Xianzhong |
Related Documents
:
|
0008802944 - Inactivation of pleiotropic regulator 1 reveals p53-dependent control of cell prolifera... 0040159084 - Key regulators in inflammation and apoptosis 0010299394 - Retrotransposition-competent human line-1 induces apoptosis in cancer cells with intact... 0036681934 - Therapeutic immunomodulation of allergic lung disease using regulatory dendritic cells ... 0003162774 - A role for stat-1 in regulating interleukin 10 production following lps challenge |
Contributors
:
|
- |
Publication Detail
:
|
Publisher : - Type : text, article Format : - |
Date Detail
:
|
2008-06-17, 2008-06-17, 2005-06-20 |
Subject
:
|
aB-crystallin, p53, Cytochrome C, Smac/Diablo, Bid, mitochondria, hydrogen peroxide, apoptosis, 610 |
Coverage
:
|
- |
Relation
:
|
Excli Journal |
Source
:
|
- |
Copyright Information
:
|
- |
Other Details
:
|
Languages : en |
Export Citation
:
|
APA/MLA Format Download EndNote Download BibTex |
Previous Document: Expression of prostacyclin-stimulating factor (PSF) in mononuclear cells of human peripheral blood a...
Next Document: Morphological and genetic characteristics of Nicotiana langsdorffii, N. glauca and its hybrid