| A core–BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes | |
Abstract/OtherAbstract
:
|
BRAF35, a structural DNA-binding protein, initially was identified as a component of a large BRCA2-containing complex. Biochemical analysis revealed the presence of a smaller core–BRAF35 complex devoid of BRCA2. Here we report the isolation of a six-subunit core–BRAF35 complex with the capacity to deacetylate histones, termed the BRAF–histone deacetylase complex (BHC), from human cells. BHC contains polypeptides reminiscent of the chromatin-remodeling complexes SWI/SNF and NuRD (nucleosome remodeling and deacetylating). Similar to NuRD, BHC contains an Mi2-like subunit, BHC80, and a PHD zinc-finger subunit as well as histone deacetylases 1/2 and an MTA-like subunit, the transcriptional corepressor CoREST. We show that BHC mediates repression of neuron-specific genes through the cis-regulatory element known as the repressor element 1 or neural restrictive silencer (RE1/NRS). Chromatin-immunoprecipitation experiments demonstrate the recruitment of BHC by the neuronal repressor REST. Expression of BRAF35 containing a single point mutation in the HMG domain of the protein abrogated REST-mediated transcriptional repression. These results demonstrate a role for core–BRAF35-containing complex in the regulation of neuron-specific genes through modulation of the chromatin structure. |
Authors
:
|
Hakimi, Mohamed-Ali, Bochar, Daniel A., Chenoweth, Josh, Lane, William S., Mandel, Gail, Shiekhattar, Ramin |
Related Documents
:
|
0008435206 - Mechanism of chromatin reassembly at the yeast pho5 promoter upon repression 0010554076 - Articles selected by faculty of 1000 biology: genetically identical snps; detailed his... 0010119636 - New late gene, dar, involved in dna replication of bacteriophage t4 i. isolation, chara... 0034296676 - Journal of molecular biology |
Contributors
:
|
- |
Publication Detail
:
|
Publisher : The National Academy of Sciences Type : Text Format : - |
Date Detail
:
|
- |
Subject
:
|
Biological Sciences |
Coverage
:
|
- |
Relation
:
|
- |
Source
:
|
- |
Copyright Information
:
|
Copyright © 2002, The National Academy of Sciences |
Other Details
:
|
Languages : en |
Export Citation
:
|
APA/MLA Format Download EndNote Download BibTex |
Previous Document: 15-Deoxy-Δ12,14-prostaglandin J2: The endogenous electrophile that induces neuronal apoptosis
Next Document: Defective lymphocyte chemotaxis in β-arrestin2- and GRK6-deficient mice