Document Detail
Simple Preparation of Large Amount of Lipopolysaccharide with Receptor Activity for Bacteriophage φXl74 from Escherichia coli C Strain
Abstract/OtherAbstract :
A convenient method for the preparation of a lipopolysaccharide (LPS) with receptor activity of bacteriophage φX174 from large amounts of the dried and defatted cells of Escherichia coli C strain was est.ablished. An improvement on bacterium cultivation led to yield over 75g of the dried and defatted cells (309g wet cells) from 6L culture medium containing 4% wt/v of D-glucose as a carbon source and 1 %wt/v of ammonium sulfate as a nitrogen source. The modified PCP method afforded 2.25g of LPS (3.0% yield from the cells) from 75g of the dried and defatted cells. It was confirmed that the obtained LPS showed in vitro an inactivation of bacteriophage φX174 from a plaque counting assay.
Authors :
Inagaki, Minoru, Kato, Muneharu, Ohsumi, Yoshiyasu, Kaitani, Koji, Nishikawa, Shiro, Kashimura, Naoki
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Contributors :
Faculty of Bioresources, Mie University, Faculty of Bioresources, Mie University, Faculty of Bioresources, Mie University, Faculty of Bioresources, Mie University, Faculty of Bioresources, Mie University, Faculty of Bioresources, Mie University, 三重大学生物資源学部生理活性化学教育研究分野, 三重大学生物資源学部生理活性化学教育研究分野, 三重大学生物資源学部生理活性化学教育研究分野, 三重大学生物資源学部生理活性化学教育研究分野, 三重大学生物資源学部生理活性化学教育研究分野, 三重大学生物資源学部生理活性化学教育研究分野, 稲垣, 穣, 加藤, 宗春, 大角, 善保, 貝谷, 康治, 西川, 司朗, 柏村, 直樹
Publication Detail :
Publisher :  Faculty of Bioresources, Mie University     Type :  Departmental Bulletin Paper / 紀要論文     Format :  391170 bytes, application/pdf    
Date Detail :
2007-05-10, 2007-05-10, 1995-12-01
Subject :
LPS, E..coli C strain, receptor, bacteriophage φX174, 491
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Languages :  ENG    
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