Document Detail
Ochratoxin A Induces Apoptotic and Necrotic Renal Cell Death
Abstract/OtherAbstract :
MDCK, LLC-PK1 and RK 13 renal cells were exposed to different OTA concentrations (2.5 to 25 μg OTA/mL of medium) for 24 hours. 2.5 μg OTA/mL significantly reduced the number of living RK 13 cells to 69.4 %, LLC-PK1 cells to 34.5 % and MDCK cells to 27.5 %, as compared to untreated control cells. Effects of OTA on cell morphology were examined by haematoxylin/ eosin or phalloidin-FITC/propidium iodide staining. 12 %, 11 % and 8.2 % apoptotic cells were observed in MDCK, LLC-PK1 and RK 13 cells treated with 2.5 μg OTA/mL, respectively. The structure of actin cytoskeleton showed significant changes in OTA-treated cells. Release of LDH into the culture medium was more abundant in MDCK and LLC-PK1 cells. OTA induced apoptotic and/or necrotic cell death in a cell type- and concentration-dependent manner. The results suggest that MDCK and LLC-PK1 cells are more sensitive to OTA than RK 13 cells.
Authors :
Petrik, József, Malić, Ana, Barišić, Karmela, Rumora, Lada, Kõszegi, Tamás, Pepeljnjak, Stjepan, Žanić-Grubišić, Tihana, Čepelak, Ivana
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Publication Detail :
Publisher :  Croatian Chemical Society     Type :  text     Format :  pdf    
Date Detail :
Subject :
ochratoxin A; apoptosis; necrosis; MDCK cells; LLC-PK1 cells; RK 13 cells
Coverage :
Relation :
Source :
Croatica Chemica Acta,, Vol.78, No.3
Copyright Information :
Parts of the contents of Croat. Chem. Acta (e. g. figures or tables) may be reproduced without prior permission, provided reference is made to their source.
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Languages :  en    
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