Document Detail
Ochratoxin A Induces Apoptotic and Necrotic Renal Cell Death
Abstract/OtherAbstract :
MDCK, LLC-PK1 and RK 13 renal cells were exposed to different OTA concentrations (2.5 to 25 μg OTA/mL of medium) for 24 hours. 2.5 μg OTA/mL significantly reduced the number of living RK 13 cells to 69.4 %, LLC-PK1 cells to 34.5 % and MDCK cells to 27.5 %, as compared to untreated control cells. Effects of OTA on cell morphology were examined by haematoxylin/ eosin or phalloidin-FITC/propidium iodide staining. 12 %, 11 % and 8.2 % apoptotic cells were observed in MDCK, LLC-PK1 and RK 13 cells treated with 2.5 μg OTA/mL, respectively. The structure of actin cytoskeleton showed significant changes in OTA-treated cells. Release of LDH into the culture medium was more abundant in MDCK and LLC-PK1 cells. OTA induced apoptotic and/or necrotic cell death in a cell type- and concentration-dependent manner. The results suggest that MDCK and LLC-PK1 cells are more sensitive to OTA than RK 13 cells.
Authors :
Petrik, József, Malić, Ana, Barišić, Karmela, Rumora, Lada, Kõszegi, Tamás, Pepeljnjak, Stjepan, Žanić-Grubišić, Tihana, Čepelak, Ivana
Related Documents :
2018548 - Toxicopathological impact of sub-lethal concentration of lead nitrate on the aerial res...
10278558 - Responses of enzymically isolated aleurone cells of oat to gibberellin a31
620378 - Construction and use of a dialysis chamber for investigating in situ the toxicity of he...
4148578 - High capacity vented nickel-cadmium cells
8322418 - Cellular mechanisms regulating follicle selection and granulosa cell differentiation in...
33649448 - Protein tyrosine phosphatase κ and shp-1 are involved in the regulation of cell-cell c...
Contributors :
-
Publication Detail :
Publisher :  Croatian Chemical Society     Type :  text     Format :  pdf    
Date Detail :
2005-09-15
Subject :
ochratoxin A; apoptosis; necrosis; MDCK cells; LLC-PK1 cells; RK 13 cells
Coverage :
-
Relation :
-
Source :
Croatica Chemica Acta, CCA@chem.pmf.hr, Vol.78, No.3
Copyright Information :
Parts of the contents of Croat. Chem. Acta (e. g. figures or tables) may be reproduced without prior permission, provided reference is made to their source.
Other Details :
Languages :  en    
Export Citation :
APA/MLA Format     Download EndNote     Download BibTex

Previous Document:  Olive and Corn Oil Enriched Diets as Modulators of Mineral Content in the Submandibular Gland during...
Next Document:  Determination of Mammillaria gracillis N-glycan Patterns by ESI Q-TOF Mass Spectrometry