Document Detail
Modulation of opioid signal transduction in SH-SY5Y neural cells by differentiating agents: Concurrent mu receptor upregulation and effector desensitization by phorbol ester
Abstract/OtherAbstract :
Long-term exposure of SH-SY5Y human neural cells to retinoic acid (RA) increased the binding of [3H]DAMGO and [3H]DPDPE by up to 3-fold compared to membranes of untreated cells. In contrast, incubation of the cells with phorbol ester (TPA) selectively up-regulated the binding of [3H]DAMGO. RA enhanced the maximal inhibition of cAMP formation in SH-SY5Y cells by both DAMGO and DPDPE from 20% for both opioids in control cells to 75% and 50%, respectively. On the other hand, the effect of TPA was limited to a marginal increase in the inhibition of cAMP formation by DAMGO. Parameters of GTP[gamma]35S binding as well as low-Km GTPase activity revealed no deterioration in the structure or function of total G protein in the TPA-treated cells, and initial Western blots showed no difference in the cell content of Go. Ongoing experiments are focusing on the covalent modification of specific G protein subtypes involved in [mu] and [delta] opioid signal transduction in SH-SY5Y cells differentiated by TPA., Peer Reviewed, http://deepblue.lib.umich.edu/bitstream/2027.42/31768/1/0000709.pdf
Authors :
Lin H.,, Carter B. D.,, Haas K. F.,, Medzihradsky F.,
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Contributors :
Dept of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Dept of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Dept of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Dept of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Publication Detail :
Publisher :  Elsevier     Type :  -     Format :  183253 bytes, 3118 bytes, application/pdf, text/plain    
Date Detail :
2006-04-10, 2006-04-10, 1994/02/21
Subject :
Public Health, Chemistry, Chemical Engineering, Biological Chemistry, Engineering, Science, Health Sciences
Coverage :
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Relation :
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Source :
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Copyright Information :
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Other Details :
Languages :  en_US    
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