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Metabolism and Recycling of N-Acetylmuramic Acid in Escherichia coli
Abstract/OtherAbstract :
Almost all bacteria possess a rigid exoskeleton (murein sacculus) that renders the cell osmotically stable. The murein heteropolymer (or peptidoglycan) is made up of glycan chains, consisting of alternating N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) subunits, interlinked by short peptide bridges. To enable the insertion of new peptidoglycan during cell elongation the existing murein is continuously degraded by cell specific lytic enzymes (autolysins). Thereby generated GlcNAc-1,6-anhydro-MurNAc-tetrapeptide is transported into the cell (turnover). Since a few years it is known that the peptide moiety is effectively re-introduced in the cell wall biosynthetic pathway, while the fate of the GlcNAc-anhydroMurNAc disaccharide remained unclear. Our hypothesis was that the recycling of anhydroMurNAc might be related to the dissimilatory pathway of MurNAc, which to date was also unknown in Escherichia coli. Therefore this thesis focussed on the investigation of the MurNAc degradation. The work presented here elucidates the MurNAc metabolic pathway and its connection to the anhydroMurNAc recycling, as well as the regulation of the genes involved.
Authors :
Jaeger, Tina
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Publisher :  Universität Konstanz, Fachbereich Biologie. Fachbereich Biologie     Type :  Thesis.Doctoral     Format :  application/pdf    
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Subject :
Zellwand, Zuckerstoffwechsel, Escherichia coli, Metabolismus, N-Acetylmuraminsäure, MurNAc, Peptidoglykan, Murein, N-Acetylmuramic acid, MurNAc, peptidoglycan, murein, cell wall, Life sciences
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Languages :  eng    
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