| Laminin-5 β3A Expression in LNCaP Human Prostate Carcinoma Cells Increases Cell Migration and Tumorigenicity1 | |
Abstract/OtherAbstract
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Interactions between extracellular matrix proteins and prostate carcinoma cells change dramatically during prostate tumor progression. We have concentrated on two key modifications that occur in the hemidesmosome in prostate carcinoma: loss of laminin-5 protein expression and altered basal cell polarity of the α6β4 integrin. We previously demonstrated two cell line-specific isoforms (β3A and β3B) of the LAMB3 message. Cells expressing only the β3B isoform did not translate the β3 protein and were unable to assemble the laminin-5 trimer. One such cell line, LNCaP, was selected to determine whether restoration of the laminin-5 β3A isoform would cause expression of a functional laminin-5 β3 chain, assembly and secretion of the laminin-5 trimer, and reversion to a non-neoplastic phenotype. Laminin-5 β3A cDNA was cloned and stably transfected into LNCaP cells. We observed the restoration of the β3 protein, but a laminin-5 trimer was not secreted. Moreover, increased cell migration was demonstrated, and tumorigenicity was increased in SCID mice. A microarray analysis, performed between transfected and nontransfected LNCaP cells, showed most changing genes to be associated with signal transduction. The β3 chain of laminin-5 may thus play an important role in signal transduction, which may enhance cell motility and tumorigenesis. |
Authors
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Calaluce, Robert, Bearss, David J, Barrera, Jean, Zhao, Yu, Han, Haiyong, Beck, Shaleen K, McDaniel, Kathy, Nagle, Ray B |
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Publisher : Neoplasia Press Inc. Type : Text Format : - |
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Copyright © 2004 Neoplasia Press, Inc. All rights reserved |
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Languages : en |
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