Document Detail
Involvement of p53-Mediated Mitochondrial Stress in the Apoptosis Induced by Flavonoids Purified from Rhus verniciflua Stokes in Human Osteosarcoma Cells
Abstract/OtherAbstract :
Dietary flavonoids have antioxidant and antitumor promoting effects. Rhus verniciflua Stokes (RVS) is a flavonoid-rich herbal medicine and has long been used as a food additive and an antitumor agent in Korea. Previous study demonstrated that a purified flavonoid fraction prepared from RVS, herein named RCMF (the RVS chloroform-methanol fraction), exhibited growth inhibition and induced apoptosis in human osteosarcoma(HOS) cells. This study evaluated if p53-mediated pathway is associated with the RCMF-induced apoptosis in HOS cells. RCMF was shown to be capable of inducing apoptosis of the cells, as expected, and transparently increased p53 expression in the cells. However, the RCMF-induced cytotoxicity was suppressed by transfecting the cells with antisense p53 oligonucleotide, which also inhibited the decrease of Bcl-2 and the increase of Bax in mitochondria, and the release of cytochrome c into cytosol. This finding suggests that p53-mediated mitochondrial stress is required for RCMF-induced apoptosis in HOS cells.
Authors :
Chung, Song-Woo, Lee, Seung-Ah, Park, Jong-Sun, Ryu, Kwon-Woo, Jang, Mun-Ju, Park, Song-Soo, Lee, Choon-Bong, Kim, Jong-Ghee, Jeon, Young-Mi, Lee, Jeong-Chae
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Publication Detail :
Publisher :  The Korean Society of Pharmacognosy     Type :  -     Format :  pdf    
Date Detail :
2008-01-29
Subject :
6154
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Languages :  en    
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