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Abstract/OtherAbstract :
Experiments were conducted to determine the effects of a treatment used to dilate the cervix for transcervical AI and embryo transfer on pregnancy or fertilization rates in sheep. Experiment 1 was conducted as a 2 x 2 factorial, with the main effects of estradiol-17b (E2) and oxytocin (OT). On d 6 of pregnancy, ewes received (i.v.) 100 mg of estradiol-17b, or saline; 10 h later, ewes received (i.v.) 400 USP units (20 mL) or saline. Blood samples were collected for progesterone analysis. Transrectal ultrasonography was used to determine pregnancy on d 25. Treatment did not affect (c 2 , P > .05) pregnancy rates. For nonpregnant ewes, the period ´ E2 ´ OT interaction (P < .01) was significant. Experiments were conducted to evaluate the effects of oxytocin and(or) cervical manipulation on fertilization rates. In Exp. 2, nulliparous ewes were assigned to one of two randomized treatment groups: 1) saline or 2) oxytocin. In Exp. 3, multiparous ewes were assigned to one of two randomized treatment groups: 1) saline-cervical manipulation or 2) oxytocin-cervical manipulation. Ewes in Exp. 2 and 3 were laparoscopically inseminated. In Exp. 2 and 3, ewes received (i.v.) either 400 USP units of oxytocin or 20 mL of saline. In Exp. 2 and 3, oocytes and(or) embryos were collected. Treatment did not affect recovery, fertilization, or pregnancy rates in Exp.2 and 3. In conclusion, the treatment used to dilate the cervix in ewes to facilitate the transcervical AI or ET does not seem to affect fertilization or pregnancy rates.
Authors :
Hensley, Erika L.
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Contributors :
Gregory S. Lewis, J. B. Hall, R. G. Saacke
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Publisher :  VT     Type :  text     Format :  application/pdf    
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Animal and Poultry Sciences
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restricted, I hereby grant to Virginia Tech or its agents the right to archive and to make available my thesis or dissertation in whole or in part in the University Libraries in all forms of media, now or hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation.
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Languages :  en    
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