| Hypoxia-inducible factor-1a mRNA contains an internal ribosome entry site that allows efficient translation during normoxia and hypoxia | |
Abstract/OtherAbstract
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Copyright © 2002 by The American Society for Cell Biology, HIF-1α is the regulated subunit of the HIF-1 transcription factor, which induces transcription of a number of genes involved in the cellular response to hypoxia. The HIF-1α protein is rapidly degraded in cells supplied with adequate oxygen but is stabilized in hypoxic cells. Using polysome profile analysis, we found that translation of HIF-1α mRNA in NIH3T3 cells is spared the general reduction in translation rate that occurs during hypoxia. To assess whether the 5'UTR of the HIF-1α mRNA contains an internal ribosome entry site (IRES), we constructed a dicistronic reporter with the HIF-1α 5'UTR inserted between two reporter coding regions. We found that the HIF-1α 5'UTR promoted translation of the downstream reporter, indicating the presence of an IRES. The IRES had activity comparable to that of the well-characterized c-myc IRES. IRES activity was not affected by hypoxic conditions that caused a reduction in cap-dependent translation, and IRES activity was less affected by serum-starvation than was cap-dependent translation. These data indicate that the presence of an IRES in the HIF-1α 5'UTR allows translation to be maintained under conditions that are inhibitory to cap-dependent translation., Kenneth J. D. Lang, Andreas Kappel, and Gregory J. Goodall |
Authors
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Lang, Kenneth John Douglas, Kappel, Andreas, Goodall, Gregory John |
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Publication Detail
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Publisher : American Society of Cell Biology Type : journal article Format : - |
Date Detail
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2006, 2006, 2002 |
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http://link.library.adelaide.edu.au/?atitle=Hypoxia-inducible%20factor-1a%20mRNA%20contains%20an%20internal%20ribosome%20entry%20site%20that%20allows%20efficient%20translation%20during%20normoxia%20and%20hypoxia&aulast=Lang&issn=1059-1524&title=Molecular%20Biology%20of%20the%20Cell&volume=13&date=2002&pages=1792-1801&spage=1792&epage=1801, http://www.molbiolcell.org/cgi/content/abstract/13/5/1792 |
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Languages : en_US |
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