Document Detail
Hinge-Bending Motion in Citrate Synthase Arising from Normal Modes Calculations
Abstract/OtherAbstract :
A normal modes analysis of the closed form of dimeric citrate synthase has been performed. The largest-amplitude collective motion predicted by this method compares well with the crystallographically observed hinge-bending motion. Such a result supports those obtained previously in the case of hinge-bending motions of smaller systems, such as lysozyme or hexokinase. Taken together, all these results suggest that low-frequency normal modes may happen to become useful for determining a first approximation of the conformational path between the closed and open forms of these proteins. Keywords: collective motion, normal modes analysis, low-frequency motion, hinge-bending, closed form of citrate synthase. Introduction In many proteins, large conformational transitions involve the relative movement of almost rigid structural elements. In citrate synthase, a two-domain protein, coenzyme A binding induces a 18 0 rotation of the small domain around an axis close to residue 274, which repre...
Authors :
Yves-henri Sanejou,Osni A. Marques,Yves-henri Sanejouand
Contributors :
The Pennsylvania State University CiteSeer Archives
Publication Detail :
Publisher :  unknown     Type :  -     Format :  ps    
Date Detail :
1995-11-17
Subject :
Yves-henri Sanejou,Osni A. Marques,Yves-henri Sanejouand Hinge-Bending Motion in Citrate Synthase Arising from Normal Modes Calculations
Coverage :
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Relation :
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Source :
http://www.cerfacs.fr/algor/reports/TR_PA_95_31.ps.gz
Copyright Information :
unrestricted
Other Details :
Languages :  en    
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