Document Detail
Heart adaptor protein 1, a novel pleckstrin homology domain family member, is required for cardiac function and left-reight asymmetry in zebrafish.
Abstract/OtherAbstract :
The emergence of a functioning heart is critical for vertebrate embryogenesis and is dependent upon a highly conserved network of molecular cues and morphogenetic events. The critical nature of heart development, as well as its complexity, is underscored by the fact that of all congenital birth defects, congenital cardiac malformations occur most often, affecting between 1 and 3% of the world's population. To gain a better understanding of cardiac development, and potentially cardiovascular disease, we analyzed the transcriptional profile of dividing, fetal cardiomyocytes and compared it to adult, quiescent cardiomyocytes. We have identified a novel, pleckstrin homology domain-containing gene, Heart Adaptor Protein 1 ( Hadp1 ) that is enriched in the developing vertebrate heart. Knockdown studies in zebrafish show hadp1 is required for cardiac contractility and calcium handling in the heart. Additionally, loss of hadp1 affects the asymmetric calcium flux around Kuppfer's vesicle and randomizes left-right asymmetry in the developing zebrafish embryo. Biochemical studies show that the PH domain of HADP1 interacts with phosphatidylinositols and HADP1 is normally localized to the plasma membrane. Collectively, these results demonstrate that Hadp1 is required for the functional output of both the heart and KV via regulation of calcium handling., Doctor of Philosophy;
Authors :
Wythe, Joshua D.
Contributors :
Urness, Lisa; Li, Dean Y.; MacRae, Calum; Jones, Christopher; Jurynee, Michael; Grunwald, David; Amack, Jeffrey; Sato, Mariko; Yost, H. Joseph;, Oncological Sciences;, School of Medicine;, University of Utah
Publication Detail :
Publisher :  University of Utah     Type :  text, dissertation;     Format :  application/pdf    
Date Detail :
2007-12
Subject :
Growth; Development; Molecular Biology;, Zebrafish; Heart; Calcium Signaling;
Coverage :
-
Relation :
Digital reproduction of “Heart adaptor protein 1, a novel pleckstrin homology domain family member, is required for cardiac function and left-reight asymmetry in zebrafish.†Spencer S. Eccles Health Sciences Library. <br><br> Print version of “Heart adaptor protein 1, a novel pleckstrin homology domain family member, is required for cardiac function and left-reight asymmetry in zebrafish.†available at J. Willard Marriott Library Special Collection. QL3.5 2007 .W98.
Source :
Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Copyright Information :
© Joshua D. Wythe.
Other Details :
Languages :  eng;    
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