| Gentisyl Alcohol Inhibits Apoptosis by Suppressing Caspase Activity Induced by Etoposide | |
Abstract/OtherAbstract
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In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide (<TEX>$10\;{\mu}g/ml$</TEX>), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with <TEX>$IC_{50}$</TEX> value of <TEX>$3.0\;{\mu}g/ml$</TEX> after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases. |
Authors
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KIM, JINHEE, KIM, DONGHYUN, KIM, MEEREE, KWON, HOJEONG, OH, TAEKWANG, LEE, CHOONGHWAN |
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Publication Detail
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Publisher : The Korean Society for Applied Microbiology and Biotechnology Type : - Format : pdf |
Date Detail
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2008-01-29 |
Subject
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66062 |
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Other Details
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Languages : en |
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