| Evaluation of <I>Salmonella</I> Gallinarum Outer Membrane Protein Based Enzyme Linked Immunosorbent Assay for Detecting Antibodies in Vaccinated and Infected Chicken | |
Abstract/OtherAbstract
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Indirect enzyme linked immunosorbent assay was developed to measure specific antibody activity to outer membrane proteins in sera and egg yolk of chickens exposed to <I>Salmonella</I> Gallinarum infection and also following vaccination. The observed end point titers were used to formulate regression equation and the absorbance data obtained at a single serum dilution were converted directly to antibody titer by subtraction method. The assay was carried out at a single serum dilution of 1:320 standardized and the antibody titers of the <I>Salmonella</I> Gallinarum infected flocks were found to be of higher magnitude when compared to vaccinated flocks. The growth-decline trend of the egg yolk antibody levels was found to be similar to that of the serum antibody levels. |
Authors
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B. Prakash, T. Suryanarayana, L. Muniyappa, G. Krishnappa |
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Contributors
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Publication Detail
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Publisher : Asian Network for Scientific Information, Pakistan Type : - Format : - |
Date Detail
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2005 |
Subject
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OMP, ELISA, Salmonella gallinarum, egg yolk, antibodies |
Coverage
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Relation
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Source
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International Journal of Poultry Science |
Copyright Information
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Other Details
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Languages : eng |
Export Citation
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