| Eradication of taro viruses from seedlings via seed rescue culture coupled with thermotherapy | |
Abstract/OtherAbstract
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In order to investigate appropriate methodologies for producing virus-free taro plants, Papua New Guinea semi-wild taro (SWT), which had been grown in a field germplasm collection of 437 cultivars and exhibited high degree of field resistance to leaf blight and high susceptibility to taro viral diseases, was used as a mother source of seeds. Natural flowering was common, to varying degree, among all cultivars. All SWT plants examined produced 4-5 flower heads per plant with an average of 15,000 potentially viable seeds per head. Seeds were grown aseptically in vitro via a seed rescue culture (SRC) technique and further maintained in an insect-proof greenhouse for a period of 8 months and regularly inspected. Plants regenerated from seeds treated at 60 C for 120 min and at 55 C for 120 min remained free of all taro viral disease symptoms. Both treatments, however, significantly reduced germination from 92.16% to 42.24% and 62.28% respectively. Six other treatments, although all reduced symptoms below those of the untreated control, failed to various degrees to eradicate the symptoms. In addition to morphological and physiological variability among seedlings, the plants showed a range of susceptibility to leaf blight. The importance of using thermotherapy with the SRC technique in taro germplasm conservation, breeding and quarantine is highlighted. |
Authors
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Wagih, M.E. |
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Contributors
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Publication Detail
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Publisher : African Crop Science Society Type : SC Format : html |
Date Detail
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1997-12-31 |
Subject
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Alomae and bobone viruses, Colocasia esculenta, dasheen mosaic, tissue culture, thermotherapy, Alomae and bobone viruses, Colocasia esculenta, dasheen mosaic, tissue culture, thermotherapy |
Coverage
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Origin of publication: Uganda |
Relation
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http://www.bioline.org.br/cs |
Source
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African Crop Science Journal (ISSN: 1021-9730) Vol 5 Num 4 |
Copyright Information
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Copyright 1997 - African Crop Science Society |
Other Details
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Languages : en |
Export Citation
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