Document Detail
Effect of mechanical agitation on hybridoma cell growth
Abstract/OtherAbstract :
Hybridoma cells (S3H5/γ2bA2) were grown in spinner flasks at different agitation speeds. It was found that cells in stationary and decline phases of growth were sensitive to shear force caused by agitation but cells in growth phase seemed less sensitive to the shear forces introduced. The death rate was found to be. 0.007 hr −1 in T flasks but 0.018 hr −1 and 0.028 hr −1 at 100 and 200 rpm, respectively, while the growth rate was about 0.05 hr −1 for all cases., Peer Reviewed, http://deepblue.lib.umich.edu/bitstream/2027.42/42280/1/10529_2005_Article_BF01024713.pdf
Authors :
Lee, Gyun Min, Huard, Thomas K., Kaminski, Mark S., Palsson, Bernhard Ø
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Contributors :
Department of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MI, USA, Department of Internal Medicine, University of Michigan, 48109, Ann Arbor, MI, USA, Department of Internal Medicine, University of Michigan, 48109, Ann Arbor, MI, USA, Department of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MI, USA, Ann Arbor
Publication Detail :
Publisher :  Kluwer Academic Publishers; Springer Science+Business Media     Type :  -     Format :  285763 bytes, 3115 bytes, application/pdf, text/plain    
Date Detail :
2006-09-08, 2006-09-08, 1988/09
Subject :
Chemistry, Biotechnology, Organic Chemistry, Bioorganic Chemistry, Biochemistry, general, Microbiology, Animal Anatomy / Morphology / Histology, Chemical Engineering, Molecular, Cellular and Developmental Biology, Chemistry, Materials Science and Engineering, Biological Chemistry, Science, Engineering, Health Sciences
Coverage :
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Source :
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Copyright Information :
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Other Details :
Languages :  en_US    
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