| Ectopic expression of PAX5 promotes self renewal of bi-phenotypic myeloid progenitors co-expressing myeloid and B-cell lineage associated genes. | |
Abstract/OtherAbstract
:
|
The transcription factor PAX5 is a critical regulator of B-cell commitment and development. Although normally not expressed in myeloid progenitors, PAX5 has recently been shown to be frequently expressed in myeloid malignancies and to suppress expression of myeloid differentiation genes, compatible with an effect on the differentiation or maintenance of myeloid progenitors. However, previous studies in which PAX5 was ectopically expressed in normal myeloid progenitors in vivo and in vitro provided conflicting results as to the effect of PAX5 on myeloid development. Herein, we demonstrate that on ectopic expression of PAX5 in bone marrow multipotent stem/progenitor cells, cells with a biphenotypic B220+GR-1/MAC-1+ phenotype are produced. These remain cytokine-dependent, but unlike control-transduced cells they sustain long-term generation of myeloid progenitors in vitro and remain capable of myeloid differentiation. Notably, PAX5+B220+GR-1/MAC-1+ myeloid progenitors coexpress, at the single-cell level, myeloid genes and otherwise B-cell–specific PAX5 target genes. These findings establish that ectopic expression of PAX5 introduces extensive self-renewal properties in otherwise short-lived myeloid progenitors. Along with the established ectopic expression of PAX5 in acute myeloid leukemia, this motivates a careful investigation of the potential involvement of ectopic PAX5 expression in myeloid and biphenotypic leukemias. |
Authors
:
|
Anderson, Kristina, Rusterholz, Corinne, Månsson, Robert, Jensen, Christina, Bacos, Karl, Zandi, Sasan, Sasaki, Yutaka, Nerlov, Claus, Sigvardsson, Mikael, Jacobsen, Sten Eirik W |
Related Documents
:
|
0009208717 - A study of the circulating myeloid progenitor cell in man / luen bik to 0029734867 - Cd45 antigen as a gating parameter for leukemias in flow cytometry utilizing a decision... 0034180317 - Characterization of the effects of p30-35 camal on normal and leukemic myelopoiesis 0033735547 - Cutaneous b-cell lymphoma : classification, prognostic factors and management recommend... |
Contributors
:
|
- |
Publication Detail
:
|
Publisher : American Society of Hematology Type : text Format : - |
Date Detail
:
|
2007 |
Subject
:
|
Medicine and Health Sciences |
Coverage
:
|
- |
Relation
:
|
- |
Source
:
|
- |
Copyright Information
:
|
- |
Other Details
:
|
Languages : eng |
Export Citation
:
|
APA/MLA Format Download EndNote Download BibTex |
Previous Document: Diet and body constitution in relation to sub-groups of breast cancer defined by tumour grade, proli...
Next Document: Non-neutral community dynamics: empirical predictions for ecosystem function and diversity from line...