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Antiproliferative and Antioxidative Activities of Methanol Extracts of Echinacea angustifolia
Abstract/OtherAbstract :
Echinacea, also blown as the purple coneflower, is a herbal medicine that has been used for centuries, customarily as a treatment for the common cold, coughs, bronchitis, upper respiratory infections, and some inflammatory conditions. We investigated the effects of methanol extracts of Echinacea angustifolia on the cytotoxicity against cancer cells <TEX>$(HepG_2,\;3LL,\;HL60,\;L1210)$</TEX> and antioxidative activity. From the test results, each part of Echinaceashowed a cytotoxic effect against the cancer cell lines, and this cytotoxic effect increased with increasing sample concentration. At 1.0 mg/mL concentration the relative cytotoxic activities of the flower bud, leaf, stern and root parts were <TEX>$90.5\%,\;52.7\%,\;37.1\%\;and\;19.2\%$</TEX>, respectively, in <TEX>$HepG_2$</TEX> cells, and <TEX>$75.5\%,\;93.3\%,\;81.2\%,\;and\;75.1\%$</TEX> respectively, in HL60 cells, as evaluated by MTT assay. <TEX>$IC_{50}(50\%\;inhibitory\;concentration)$</TEX> of the methanol extracts of the Echinacea flower bud was 0.214 mg/mL on <TEX>/<TEX>$HepG_2$</TEX> cells, and that of the Echinacea leaf and root was 0.166 mg/mL and 0.210 mg/mL, respectively, on HL60 cells. After <TEX>/$</TEX>HepG_2$&lt;/TEX&gt; cells were incubated for 6 days at <TEX>$37^{\circ}C$</TEX> with various concentrations of each part, the cell number increased while the inhibition rate on the <TEX>/$HepG_2$</TEX> cell growth decreased. The antioxidative activities of the flower bud, leaf, stem and root parts were <TEX>$59.0\%$</TEX> (0.75 mg/mL), <TEX>$80.76\%$</TEX> (0.5 mg/mL), <TEX>$95.5\%$</TEX> (0.25mg/mL) and <TEX>$98.15\%$</TEX> (0.25 mg/mL), respectively, as evaluated by electron donating ability. These results indicated that Echinacea angustifolia has strong anticancer and antioxidative effects in vitro. Echinacea angustifolia의 부위별(꽃봉오리, 잎, 줄기 및 뿌리) 메탄을 추출물의 암세포(HepG2, 3LL, HL60, L1210)를 대상으로 한 항암 활성과 전자공여능을 검색한 결과는 다음과 같다. 1. 에키네시아 메탄을 추출물의 간암세포인 HepG2 cell 대한 MTT assay는 농도의존적으로 세포독성 효과가 증가하였으며, 인간유래 백혈암세포인 HL60 cell의 경우에 잎과 뿌리 추출물은 저농도에서부터 독성효과가 컸고, 줄기와 꽃봉오리는 저농도에서는 독성효과가 낮았으나 고농도로 갈수록 독성효과가 커짐을 알 수 있었다 폐암세포인 3LL cell과 마우스 유래 백혈암세포인 L1210 cell에 대한 경우는 세포독성효과가 없었다. 2. Hemacytometer에 의한 HepG2 cell의 암세포 성장에 미치는 효과는 배양기간이 증가함에 따라 농도의존적으로 증식억제 효과가 증가되었다. 3. HepG2 세포주의 형태학적 변화에서 대조군은 암세포가 조밀하게 중첩되어 증식되었으나 시료를 0.5 mg/mL 이상의 농도로 첨가하였을 때 세포의 결속력이 감소되어 세포주위가 흐트러지고 세포가 사멸된 것을 관찰 할 수가 있었다. 4. 전자공여능의 수준은 부위에 따라 최고의 EDA를 나타내는 농도가 달랐으며, 뿌리와 줄기부위는 저농도에서도 매우 높은 전자공여능을 나타내었다.
Authors :
Lee, Joon-Kyoung, Koo, Seung-Ja
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Publisher :  Korean Society of Food and Cookery Science     Type :  -     Format :  pdf    
Date Detail :
2008-01-29
Subject :
6415
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