Document Detail

A zinc-resistant human epithelial cell line is impaired in cadmium and manganese import.
MedLine Citation:
PMID:  18433815     Owner:  NLM     Status:  MEDLINE    
A human epithelial cell line (HZR) growing with high zinc concentrations has been analyzed for its ability to sustain high cadmium concentrations. Exposure to up to 200 microM of cadmium acetate for 24 h hardly impacted viability, whereas most of parental HeLa cells were killed by less than 10 microM of cadmium. Upon challenge by 35 fold higher cadmium concentrations than HeLa cells, HZR cells did not display increased DNA damage, increased protein oxidation, or changed intracellular cadmium localization. Rather, the main cause of resistance against cadmium was by avoiding cadmium entry into cells, which differs from that against zinc as the latter accumulates inside cells. The zinc-resistant phenotype of these cells was shown to also impair extracellular manganese uptake. Manganese and cadmium competed for entry into HeLa cells. Probing formerly identified cadmium or manganese transport systems in different animal cells did not evidence any significant change between HeLa and HZR cells. These results reveal zinc adaptation influences manganese and cadmium cellular traffic and they highlight previously unknown connections among homeostasis of divalent metals.
Estelle Rousselet; Pierre Richaud; Thierry Douki; Jocelyne Garcia Chantegrel; Alain Favier; Alexandre Bouron; Jean-Marc Moulis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-06
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  230     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-15     Completed Date:  2008-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  312-9     Citation Subset:  IM    
CEA, DSV, IRTSV, Laboratoire de Chimie et Biologie des Métaux, Grenoble, France.
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MeSH Terms
Biological Transport
Cadmium / pharmacokinetics*,  toxicity
Drug Resistance
Epithelial Cells / drug effects
Glutathione / physiology
Hela Cells
Manganese / pharmacokinetics*
Zinc / pharmacology*
Reg. No./Substance:
70-18-8/Glutathione; 7439-96-5/Manganese; 7440-43-9/Cadmium; 7440-66-6/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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