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The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast.
MedLine Citation:
PMID:  23311928     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Fission yeast flocculates non-sexually by induction of the flocculin encoded by gsf2(+) which is controlled by the positive regulator Mbx2. Here we report a novel gene designated gsf1(+) found to be a negative regulator of non-sexual flocculation. We identified gsf1(+) as a multicopy suppressor of a sam2 mutation, which caused growth sensitivity to Ca(2+) and also found a nonsense mutation in gsf1(+) in a previously isolated gsf1 mutant. The gsf1(+) gene encodes a 547 aa protein containing a Zn(2)-Cys(6) binuclear cluster type Zinc finger motif. The Gsf1 protein localized in the nucleus, consistent with a role as a transcription factor. Deletion of gsf1(+) resulted in non-sexual flocculation inducible by CaCl(2) , which was suppressed by addition of EDTA or galactose. Both gsf2(+) and mbx2(+) were highly expressed in the gsf1 mutant. gsf1∆ gsf2∆ and gsf1∆ mbx2∆ double mutants did not flocculate, suggesting that gsf1(+) is an upstream regulator. In addition, the gsf1 mutant was sensitive to CaCl(2) , KCl, HU and TBZ, consistent with the possibility that gsf1(+) plays a role in functions unrelated to flocculation. Taken together, these results suggest that nonsexual flocculation in fission yeast is negatively controlled by Gsf1 which controls expression of mbx2(+) and gsf2(+) . © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Tomohiko Matsuzawa; Youko Kageyama; Kazuyoshi Ooishi; Makoto Kawamukai; Kaoru Takegawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-13
Journal Detail:
Title:  FEMS yeast research     Volume:  -     ISSN:  1567-1364     ISO Abbreviation:  FEMS Yeast Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Department of Bioscience & Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan.
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