| The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast. | |
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MedLine Citation:
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PMID: 23311928 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Fission yeast flocculates non-sexually by induction of the flocculin encoded by gsf2(+) which is controlled by the positive regulator Mbx2. Here we report a novel gene designated gsf1(+) found to be a negative regulator of non-sexual flocculation. We identified gsf1(+) as a multicopy suppressor of a sam2 mutation, which caused growth sensitivity to Ca(2+) and also found a nonsense mutation in gsf1(+) in a previously isolated gsf1 mutant. The gsf1(+) gene encodes a 547 aa protein containing a Zn(2)-Cys(6) binuclear cluster type Zinc finger motif. The Gsf1 protein localized in the nucleus, consistent with a role as a transcription factor. Deletion of gsf1(+) resulted in non-sexual flocculation inducible by CaCl(2) , which was suppressed by addition of EDTA or galactose. Both gsf2(+) and mbx2(+) were highly expressed in the gsf1 mutant. gsf1∆ gsf2∆ and gsf1∆ mbx2∆ double mutants did not flocculate, suggesting that gsf1(+) is an upstream regulator. In addition, the gsf1 mutant was sensitive to CaCl(2) , KCl, HU and TBZ, consistent with the possibility that gsf1(+) plays a role in functions unrelated to flocculation. Taken together, these results suggest that nonsexual flocculation in fission yeast is negatively controlled by Gsf1 which controls expression of mbx2(+) and gsf2(+) . © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
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Authors:
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Tomohiko Matsuzawa; Youko Kageyama; Kazuyoshi Ooishi; Makoto Kawamukai; Kaoru Takegawa |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-13 |
Journal Detail:
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Title: FEMS yeast research Volume: - ISSN: 1567-1364 ISO Abbreviation: FEMS Yeast Res. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101085384 Medline TA: FEMS Yeast Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Department of Bioscience & Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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