| The zinc-binding fragment of HypA from Helicobacter pylori: a tempting site also for nickel ions. | |
| | |
MedLine Citation:
|
PMID: 23338727 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
HypA, a nickel accessory protein from H. pylori, binds a zinc ion in it's structural site, a loop with two conserved CXXC motifs (Ac-ELECKDCSHVFKPNALDYGVCEKCHS-NH(2)). There are at least three hypotheses on the binding mode of this ion. In this paper, we try to understand how Zn(2+) binds to this fragment and why Ni(2+), a metal with quite a high affinity towards thiolic sites, doesn't compete with zinc in the binding to this motif. Potentiometric titrations, mass spectrometry, NMR, UV-Vis and CD spectroscopy help us to compare the coordination modes in both metal complexes and discuss their thermodynamic stabilities. |
| | |
Authors:
|
Magdalena Rowinska-Zyrek; Slawomir Potocki; Danuta Witkowska; Daniela Valensin; Henryk Kozlowski |
Related Documents
:
|
12634967 - Bilateral increase in striatal dopamine d2 receptor density in the 6-hydroxydopamine-le... 9590177 - Tip47: a cargo selection device for mannose 6-phosphate receptor trafficking. 9865517 - Nature of methamphetamine-induced rapid and reversible changes in dopamine transporters. 7626647 - Rapid substrate translocation by the multisubunit, erythroid glucose transporter requir... 15475297 - Graal: a drosophila gene coding for several mosaic serine proteases. 7881727 - Characterization of beta 1- and beta 3-adrenoceptors in intact brown adipocytes of the ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-21 |
Journal Detail:
|
Title: Dalton transactions (Cambridge, England : 2003) Volume: - ISSN: 1477-9234 ISO Abbreviation: Dalton Trans Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-22 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101176026 Medline TA: Dalton Trans Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland. henryk.kozlowski@chem.uni.wroc.pl. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: PCSK9 Inhibitors: Potential in Cardiovascular Therapeutics.
Next Document: Folic acid supplementation and cancer risk: A meta-analysis of randomized controlled trials.