Document Detail

The yeast potassium transporter TRK2 is able to substitute for TRK1 in its biological function under low K and low pH conditions.
MedLine Citation:
PMID:  16823886     Owner:  NLM     Status:  MEDLINE    
In S. cerevisiae, K+ transport relies principally on two structurally related membrane proteins, known as Trk1p and Trk2p. Direct involvement in cation movements has been demonstrated for Trk1p, which is a high-affinity K+ transporter. Initially described as a low-affinity K+ transporter, Trk2p seems to play a minor role in K+ transport, since its activity is only apparent under very specific conditions, such as in a Deltasin3 background. Here we show that growth of a Deltatrk1Deltasin3 double mutant, under K+-limiting conditions or at low pH, is Trk2p-dependent, and by Northern blot analysis we demonstrate that deletion of SIN3 results in transcriptional derepression of TRK2. In addition, we show that heterologous overexpression of TRK2 with the inducible GAL1 promoter bypasses Sin3p repression in a Deltatrk1Deltatrk2 double mutant and fully restores growth under non-permissive conditions. Furthermore, kinetic experiments in a Deltatrk1Deltasin3 double mutant revealed a K+ transporter with an apparent high affinity and a moderate capacity. Taken together, these results indicate that TRK2 encodes a functional K+ transporter that, under our experimental conditions, displays distinctive kinetic characteristics.
Bertha Michel; Carlos Lozano; Miriam Rodríguez; Roberto Coria; Jorge Ramírez; Antonio Peña
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  23     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-07-12     Completed Date:  2006-09-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  England    
Other Details:
Languages:  eng     Pagination:  581-9     Citation Subset:  IM    
Copyright Information:
Copyright 2006 John Wiley & Sons, Ltd.
Depto de Genética Molecular, Instituto de Fisiología Celular, UNAM, Circuito Exterior s/n, Ciudad Universitaria, D.F. 04510, México.
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MeSH Terms
Blotting, Northern
Cation Transport Proteins / biosynthesis,  genetics,  metabolism*
Chlorides / pharmacokinetics
DNA, Fungal / genetics
Gene Deletion
Histone Deacetylases
Hydrogen-Ion Concentration
Ion Transport
Mutagenesis, Insertional
Polymerase Chain Reaction
Potassium / metabolism*
RNA, Messenger / biosynthesis,  genetics
Repressor Proteins / genetics,  metabolism
Rubidium / pharmacokinetics
Rubidium Radioisotopes
Saccharomyces cerevisiae / genetics,  metabolism*
Saccharomyces cerevisiae Proteins / biosynthesis,  genetics,  metabolism*
Transcription Factors / deficiency,  genetics,  metabolism
Reg. No./Substance:
0/Cation Transport Proteins; 0/Chlorides; 0/DNA, Fungal; 0/RNA, Messenger; 0/Repressor Proteins; 0/Rubidium Radioisotopes; 0/SIN3 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/TRK2 protein, S cerevisiae; 0/Transcription Factors; 136956-54-2/TRK1 protein, S cerevisiae; 7440-09-7/Potassium; 7440-17-7/Rubidium; 7791-11-9/rubidium chloride; EC Deacetylases

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