Document Detail

The yeast kinase Swe1 is required for proper entry into cell cycle after arrest due to ribosome biogenesis and protein synthesis defects.
MedLine Citation:
PMID:  15107621     Owner:  NLM     Status:  MEDLINE    
Sda1 is an essential protein required for cell cycle progression in Saccharomyces cerevisiae. Here, we show that the sda1-1 mutation causes a defect in the formation and nuclear export of 60S ribosomal subunits. Moreover, the sda1-1, but also other mutants defective in ribosome biogenesis (e.g., rix1-1 and tif6Delta), exhibit a G1 arrest, which could be the consequence of impaired ribosome biogenesis. Interestingly, additional deletion of the non-essential Swe1 kinase, the homolog of S. pombe Wee1, causes a pronounced delay in entering a new cell cycle in sda1-1, rix1-1 and tif6Delta cells, when shifted back from restrictive to permissive conditions. However, such a prolonged delay is independent of the Tyr19 phosphorylation in Cdc28. Moreover, the lack of Swe1 causes delay in budding and DNA replication in cells released from the G1 arrest due to the block of protein synthesis. Our data suggest that Swe1 is required for timely entry into cell cycle after a G1 arrest caused by impairment in pre-60S biogenesis and in protein synthesis. Therefore we propose that Swe1, which is required for coordination of cell growth and cell division in G2/M, also has a role in the beginning of the cell cycle.
Francesca Saracino; Jochen Bassler; Diego Muzzini; Ed Hurt; Maria Luisa Agostoni Carbone
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-05-15
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  3     ISSN:  1538-4101     ISO Abbreviation:  Cell Cycle     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-21     Completed Date:  2004-10-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  648-54     Citation Subset:  IM    
Dipartimento di Scienze Biomolecolari e Biotecnologie, Università di Milano, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
CDC28 Protein Kinase, S cerevisiae / metabolism
Cell Cycle / physiology*
Cell Cycle Proteins / genetics,  metabolism
Nuclear Proteins / genetics,  metabolism
Protein Biosynthesis
Protein-Tyrosine Kinases / genetics,  metabolism*
Ribosomes / metabolism*
Saccharomyces cerevisiae / genetics,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Nuclear Proteins; 0/SDA1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC 2.7.1.-/SWE1 protein, S cerevisiae; EC Kinases; EC Protein Kinase, S cerevisiae

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  ErbB2 and TGF-beta: a cooperative role in mammary tumor progression?
Next Document:  Control of tumor suppressor p53 function by endoplasmic reticulum stress.