| The yeast DNA damage checkpoint proteins control a cytoplasmic response to DNA damage. | |
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MedLine Citation:
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PMID: 17586685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A single HO endonuclease-induced double-strand break (DSB) is sufficient to activate the DNA damage checkpoint and cause Saccharomyces cells to arrest at G(2)/M for 12-14 h, after which cells adapt to the presence of the DSB and resume cell cycle progression. The checkpoint signal leading to G(2)/M arrest was previously shown to be nuclear-limited. Cells lacking ATR-like Mec1 exhibit no DSB-induced cell cycle delay; however, cells lacking Mec1's downstream protein kinase targets, Rad53 or Chk1, still have substantial G(2)/M delay, as do cells lacking securin, Pds1. This delay is eliminated only in the triple mutant chk1Delta rad53Delta pds1Delta, suggesting that Rad53 and Chk1 control targets other than the stability of securin in enforcing checkpoint-mediated cell cycle arrest. The G(2)/M arrest in rad53Delta and chk1Delta revealed a unique cytoplasmic phenotype in which there are frequent dynein-dependent excursions of the nucleus through the bud neck, without entering anaphase. Such excursions are infrequent in wild-type arrested cells, but have been observed in cells defective in mitotic exit, including the semidominant cdc5-ad mutation. We suggest that Mec1-dependent checkpoint signaling through Rad53 and Chk1 includes the repression of nuclear movements that are normally associated with the execution of anaphase. |
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Authors:
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Farokh Dotiwala; Julian Haase; Ayelet Arbel-Eden; Kerry Bloom; James E Haber |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-06-22 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 104 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-04 Completed Date: 2007-09-26 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 11358-63 Citation Subset: IM |
Affiliation:
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Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454-9110, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytoplasm
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physiology* DNA Breaks, Double-Stranded* DNA Repair / physiology* Genes, cdc / physiology* Microtubule-Associated Proteins / genetics Saccharomyces cerevisiae / cytology, genetics*, physiology Saccharomyces cerevisiae Proteins / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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GM32238/GM/NIGMS NIH HHS; GM61799/GM/NIGMS NIH HHS; R01 GM032238-18/GM/NIGMS NIH HHS; R01 GM032238-20/GM/NIGMS NIH HHS; R01 GM061766-07/GM/NIGMS NIH HHS; R01 GM061766-08/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Microtubule-Associated Proteins; 0/Saccharomyces cerevisiae Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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