| A yeast BH3-only protein mediates the mitochondrial pathway of apoptosis. | |
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MedLine Citation:
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PMID: 21673659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondrial outer membrane permeabilization is a watershed event in the process of apoptosis, which is tightly regulated by a series of pro- and anti-apoptotic proteins belonging to the BCL-2 family, each characteristically possessing a BCL-2 homology domain 3 (BH3). Here, we identify a yeast protein (Ybh3p) that interacts with BCL-X(L) and harbours a functional BH3 domain. Upon lethal insult, Ybh3p translocates to mitochondria and triggers BH3 domain-dependent apoptosis. Ybh3p induces cell death and disruption of the mitochondrial transmembrane potential via the mitochondrial phosphate carrier Mir1p. Deletion of Mir1p and depletion of its human orthologue (SLC25A3/PHC) abolish stress-induced mitochondrial targeting of Ybh3p in yeast and that of BAX in human cells, respectively. Yeast cells lacking YBH3 display prolonged chronological and replicative lifespans and resistance to apoptosis induction. Thus, the yeast genome encodes a functional BH3 domain that induces cell death through phylogenetically conserved mechanisms. |
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Authors:
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Sabrina Büttner; Doris Ruli; F-Nora Vögtle; Lorenzo Galluzzi; Barbara Moitzi; Tobias Eisenberg; Oliver Kepp; Lukas Habernig; Didac Carmona-Gutierrez; Patrick Rockenfeller; Peter Laun; Michael Breitenbach; Chamel Khoury; Kai-Uwe Fröhlich; Gerald Rechberger; Chris Meisinger; Guido Kroemer; Frank Madeo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-06-14 |
Journal Detail:
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Title: The EMBO journal Volume: 30 ISSN: 1460-2075 ISO Abbreviation: EMBO J. Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-07-20 Completed Date: 2011-09-29 Revised Date: 2011-12-22 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
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Languages: eng Pagination: 2779-92 Citation Subset: IM |
Affiliation:
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Institute of Molecular Biosciences, University of Graz, Graz, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Apoptosis* Apoptosis Regulatory Proteins / antagonists & inhibitors, genetics, metabolism* Blotting, Western Bone Neoplasms / genetics, metabolism Cell Cycle Flow Cytometry Humans Immunoprecipitation Lung Neoplasms / genetics, metabolism Membrane Potential, Mitochondrial Mice Mitochondria / genetics, metabolism* Mitochondrial Proteins / genetics, metabolism Molecular Sequence Data Peptide Fragments / pharmacology* Phosphate Transport Proteins / genetics, metabolism Proto-Oncogene Proteins / pharmacology* RNA, Messenger / genetics RNA, Small Interfering / genetics Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae / genetics, metabolism* Saccharomyces cerevisiae Proteins / antagonists & inhibitors, genetics, metabolism* Sequence Homology, Amino Acid Signal Transduction* Tumor Cells, Cultured bcl-2-Associated X Protein / genetics, metabolism bcl-X Protein / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/Bax protein (53-86); 0/MIR1 protein, S cerevisiae; 0/Mitochondrial Proteins; 0/Peptide Fragments; 0/Phosphate Transport Proteins; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/RNA, Small Interfering; 0/Saccharomyces cerevisiae Proteins; 0/bcl-2-Associated X Protein; 0/bcl-X Protein |
| Comments/Corrections | |
Comment In:
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EMBO J. 2011 Jul 20;30(14):2754-6
[PMID:
21772325
]
Cell Death Differ. 2011 Oct;18(10):1543-4 [PMID: 21909117 ] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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