| Characterization of a family GH5 xylanase with activity on neutral oligosaccharides and evaluation as a pulp bleaching aid. | |
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MedLine Citation:
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PMID: 20656870 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for methyl-glucuronic acid side chains for catalysis. The enzyme was evaluated on Eucalyptus kraft pulp, showing its effectiveness as a bleaching aid. |
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Authors:
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Oscar Gallardo; María Fernández-Fernández; Cristina Valls; Susana Valeria Valenzuela; M Blanca Roncero; Teresa Vidal; Pilar Díaz; F I Javier Pastor |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-23 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-08 Completed Date: 2011-01-18 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 6290-4 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/HM594284 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacillus / enzymology* Base Sequence Bleaching Agents / metabolism* Chromatography, Gel Chromatography, Ion Exchange Chromatography, Liquid Chromatography, Thin Layer Cloning, Molecular Eucalyptus / chemistry Hydrogen-Ion Concentration Lignin / metabolism Molecular Sequence Data Oligosaccharides / metabolism* Sequence Analysis, DNA Temperature Xylosidases / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bleaching Agents; 0/Oligosaccharides; 8068-05-1/Kraft lignin; 9005-53-2/Lignin; EC 3.2.1.-/Xylosidases |
| Comments/Corrections | |
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