| xCt cystine transporter expression in HEK293 cells: pharmacology and localization. | |
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MedLine Citation:
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PMID: 11302733 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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xCT, the core subunit of the system x(c)(-) high affinity cystine transporter, belongs to a superfamily of glycoprotein-associated amino acid transporters. Although xCT was shown to promote cystine transport in Xenopus oocytes, little work has been done with mammalian cells (Sato, H., Tamba, M., Ishii, T., and Bannai, S. J. Biol. Chem. 274, 11455-11458, 1999). Therefore, we have constructed mammalian expression vectors for murine xCT and its accessory subunit 4F2hc and transfected them into HEK293 cells. We report that this transporter binds cystine with high affinity (81 microM) and displays a pharmacological profile expected for system x(c)(-). Surprisingly, xCT transport activity in HEK293 cells is not dependent on the co-expression of the exogenous 4F2hc. Expression of GFP-tagged xCT indicated a highly clustered plasma membrane and intracellular distribution suggesting the presence of subcellular domains associated with combating oxidative stress. Our results indicate that HEK293 cells transfected with the xCT subunit would be a useful vehicle for future structure-function and pharmacology experiments involving system x(c)(-). |
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Authors:
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A Y Shih; T H Murphy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 282 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-04-16 Completed Date: 2001-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1132-7 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Department of Psychiatry, University of British Columbia, 4N1-2255 Wesbrook Mall, Vancouver, British Columbia, V6T 1Z3, Canada. thmurphy@interchange.ubc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Transport System y+* Animals Antigens, CD / biosynthesis, genetics Antigens, CD98 Biological Transport / physiology Carrier Proteins / biosynthesis*, genetics Cell Line Cell Membrane / metabolism Cystine / metabolism Genetic Vectors / genetics, metabolism Glutamic Acid / pharmacokinetics Green Fluorescent Proteins Humans Intracellular Fluid / metabolism Kidney / cytology, metabolism* Luminescent Proteins / genetics Mice Oxidative Stress / physiology Protein Subunits Recombinant Fusion Proteins / biosynthesis, genetics Transfection |
| Chemical | |
Reg. No./Substance:
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0/Amino Acid Transport System y+; 0/Antigens, CD; 0/Antigens, CD98; 0/Carrier Proteins; 0/Luminescent Proteins; 0/Protein Subunits; 0/Recombinant Fusion Proteins; 0/SLC7A11 protein, human; 0/Slc7a11 protein, mouse; 147336-22-9/Green Fluorescent Proteins; 56-86-0/Glutamic Acid; 56-89-3/Cystine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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