| The worker honeybee fat body proteome is extensively remodeled preceding a major life-history transition. | |
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MedLine Citation:
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PMID: 21969861 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Honeybee workers are essentially sterile female helpers that make up the majority of individuals in a colony. Workers display a marked change in physiology when they transition from in-nest tasks to foraging. Recent technological advances have made it possible to unravel the metabolic modifications associated with this transition. Previous studies have revealed extensive remodeling of brain, thorax, and hypopharyngeal gland biochemistry. However, data on changes in the abdomen is scarce. To narrow this gap we investigated the proteomic composition of abdominal tissue in the days typically preceding the onset of foraging in honeybee workers. In order to get a broader representation of possible protein dynamics, we used workers of two genotypes with differences in the age at which they initiate foraging. This approach was combined with RNA interference-mediated downregulation of an insulin/insulin-like signaling component that is central to foraging behavior, the insulin receptor substrate (irs), and with measurements of glucose and lipid levels. Our data provide new insight into the molecular underpinnings of phenotypic plasticity in the honeybee, invoke parallels with vertebrate metabolism, and support an integrated and irs-dependent association of carbohydrate and lipid metabolism with the transition from in-nest tasks to foraging. |
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Authors:
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Queenie W T Chan; Navdeep S Mutti; Leonard J Foster; Sarah D Kocher; Gro V Amdam; Florian Wolschin |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-09-28 |
Journal Detail:
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Title: PloS one Volume: 6 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2011 |
Date Detail:
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Created Date: 2011-10-04 Completed Date: 2012-04-13 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e24794 Citation Subset: IM |
Affiliation:
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Centre for High-Throughput Biology and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abdomen
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physiology Animals Bees / genetics*, physiology* Carbohydrates / chemistry Down-Regulation Female Gene Expression Regulation* Genotype Glucose / metabolism Insulin / metabolism Lipid Metabolism Lipids / chemistry Models, Genetic Phenotype Proteome Proteomics / methods* RNA Interference RNA, Double-Stranded / metabolism RNA, Messenger / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P01 AG22500/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Insulin; 0/Lipids; 0/Proteome; 0/RNA, Double-Stranded; 0/RNA, Messenger; 50-99-7/Glucose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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