Document Detail

A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria.
Jump to Full Text
MedLine Citation:
PMID:  21085179     Owner:  NLM     Status:  MEDLINE    
Bacteria have developed mechanisms to communicate and compete with one another in diverse environments. A new form of intercellular communication, contact-dependent growth inhibition (CDI), was discovered recently in Escherichia coli. CDI is mediated by the CdiB/CdiA two-partner secretion (TPS) system. CdiB facilitates secretion of the CdiA 'exoprotein' onto the cell surface. An additional small immunity protein (CdiI) protects CDI(+) cells from autoinhibition. The mechanisms by which CDI blocks cell growth and by which CdiI counteracts this growth arrest are unknown. Moreover, the existence of CDI activity in other bacteria has not been explored. Here we show that the CDI growth inhibitory activity resides within the carboxy-terminal region of CdiA (CdiA-CT), and that CdiI binds and inactivates cognate CdiA-CT, but not heterologous CdiA-CT. Bioinformatic and experimental analyses show that multiple bacterial species encode functional CDI systems with high sequence variability in the CdiA-CT and CdiI coding regions. CdiA-CT heterogeneity implies that a range of toxic activities are used during CDI. Indeed, CdiA-CTs from uropathogenic E. coli and the plant pathogen Dickeya dadantii have different nuclease activities, each providing a distinct mechanism of growth inhibition. Finally, we show that bacteria lacking the CdiA-CT and CdiI coding regions are unable to compete with isogenic wild-type CDI(+) cells both in laboratory media and on a eukaryotic host. Taken together, these results suggest that CDI systems constitute an intricate immunity network with an important function in bacterial competition.
Stephanie K Aoki; Elie J Diner; Claire T'kint de Roodenbeke; Brandt R Burgess; Stephen J Poole; Bruce A Braaten; Allison M Jones; Julia S Webb; Christopher S Hayes; Peggy A Cotter; David A Low
Related Documents :
20729849 - Colour-barcoded magnetic microparticles for multiplexed bioassays.
24162519 - The evolution of degenerative marrow (modic) changes in the cervical spine in neck pain...
391109 - Comparative serologic study of equine piroplasmosis, with card and complement-fixation ...
1680659 - In vitro glucuronidation of 3'-azido-3'-deoxythymidine by human liver. role of udp-gluc...
20219239 - In vitro evaluation of fresh sperm quality in tomcats: a comparison of two collection t...
138679 - Structure and function of the two heads of the myosin molecule. iii. cooperativity of t...
19896789 - Mri for the size assessment of pure ductal carcinoma in situ (dcis): a prospective stud...
16854609 - Optimal control of set-up margins and internal margins for intra- and extracranial radi...
8372629 - Peripheral autonomic involvement in the carpal tunnel syndrome.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nature     Volume:  468     ISSN:  1476-4687     ISO Abbreviation:  Nature     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-18     Completed Date:  2011-01-06     Revised Date:  2013-12-23    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  England    
Other Details:
Languages:  eng     Pagination:  439-42     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Bacterial Toxins / chemistry,  genetics,  immunology,  metabolism*
Contact Inhibition / immunology,  physiology
Enterobacteriaceae / enzymology,  genetics,  metabolism
Escherichia coli Proteins / antagonists & inhibitors,  chemistry,  genetics,  metabolism
Membrane Proteins / antagonists & inhibitors,  chemistry,  genetics,  metabolism
Molecular Sequence Data
Uropathogenic Escherichia coli / enzymology,  genetics,  growth & development,  metabolism*
Grant Support
AI043986/AI/NIAID NIH HHS; GM078634/GM/NIGMS NIH HHS; R01 GM078634/GM/NIGMS NIH HHS; U54 AI065359/AI/NIAID NIH HHS; U54 AI065359-056074/AI/NIAID NIH HHS; U54 AI065359-066074/AI/NIAID NIH HHS; U54 AI065359-07/AI/NIAID NIH HHS; U54AI065359/AI/NIAID NIH HHS
Reg. No./Substance:
0/Bacterial Toxins; 0/CdiA protein, E coli; 0/CdiB protein, E coli; 0/CdiI protein, E coli; 0/Escherichia coli Proteins; 0/Membrane Proteins
Comment In:
Nat Rev Microbiol. 2011 Jan;9(1):3   [PMID:  21204301 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-journal-id): 0410462
Journal ID (pubmed-jr-id): 6011
Journal ID (nlm-ta): Nature
ISSN: 0028-0836
ISSN: 1476-4687
Article Information
Download PDF

License:Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:
nihms-submitted publication date: Day: 30 Month: 9 Year: 2010
Print publication date: Day: 18 Month: 11 Year: 2010
pmc-release publication date: Day: 18 Month: 5 Year: 2011
Volume: 468 Issue: 7322
First Page: 439 Last Page: 442
ID: 3058911
PubMed Id: 21085179
DOI: 10.1038/nature09490
ID: nihpa234506
U54 AI065359-07 ||AI
U54 AI065359-066074 ||AI
U54 AI065359-056074 ||AI
National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID

A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria
Stephanie K. Aoki Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Elie J. Diner Affiliation: Biomolecular Science and Engineering Program, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Claire t'Kint de Roodenbeke Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Brandt R. Burgess Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Stephen J. Poole Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Bruce A. Braaten Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Allison M. Jones Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Julia S. Webb Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Christopher S. Hayes Affiliation: Biomolecular Science and Engineering Program, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Peggy A. Cotter* Affiliation: Biomolecular Science and Engineering Program, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
David A. Low Affiliation: Biomolecular Science and Engineering Program, University of California–Santa Barbara (UCSB), Santa Barbara, CA 93106-9625, USA
Correspondence: Correspondence and requests for materials should be addressed to D.A.L. (
*Current address: Department of Microbiology and Immunology, University of North Carolina - Chapel Hill School of Medicine, Chapel Hill, NC 27599-7290
Co-contributing senior authors

Contact-dependent growth inhibition (CDI) is a phenomenon discovered in Escherichia coli strain EC93, which inhibits the growth of other E. coli strains upon direct cell-to-cell contact 2. CDI is mediated by the CdiB/CdiA two-partner secretion (TPS) system. CdiB is required for assembly of the CdiA ‘exoprotein’ onto the outer membrane. Epitope insertion mutagenesis revealed the importance of the CdiA C-terminus (CdiA-CT) in CDI 2. Genetic and antibody blocking experiments identified BamA, an essential protein required for outer membrane biogenesis, as the CDI receptor on target cells 3. The inner membrane multidrug transporter AcrB may also play a role as acrB mutants, like bamA mutants, are resistant to CDI 3. For EC93 mediated CDI, growth inhibition coincides with dissipation of the proton motive force across the cytoplasmic membrane, decreased aerobic respiration, and decreased ATP levels in the target cells 4. EC93 is protected from autoinhibition by a small immunity protein (CdiI), which is encoded immediately downstream of cdiA2. These data suggest that CdiA binds BamA and delivers a signal, possibly a CdiA-derived toxin, which then inhibits target cell growth. CdiI could confer immunity to cells by binding to the CdiA peptide or otherwise neutralizing the growth inhibitory signal (Supplementary Fig.1a).

Previous complementation analysis indicated the presence of functional cdiB and cdiA homologues in uropathogenic E. coli (UPEC), but no cdiI homolog was identified 2, 5. Inspection of the cdi locus from E. coli UPEC 536 revealed a small open reading frame (ORF) in the same relative location as, but lacking significant sequence identity to, cdiIEC93. Expression of this ORF (cdiI536) protected E. coli from growth inhibition mediated by cells expressing CdiA536, but not from cells that express CdiAEC93 (Fig. 1a). Similarly, CdiIEC93 only provided immunity to cells expressing CdiAEC93 (Fig. 1a). Therefore, the protection conferred by CdiI appears to be limited to its cognate CDI system. Alignment of CdiAEC93 and CdiA536 showed that the N-terminal ∼3,000 residues (up to and including a common VENN peptide motif) are 78% identical, but the C-terminal ∼220 residues share no significant similarity 6 (Fig. 1b). To determine if the dissimilar C-termini of CdiAEC93 and CdiA536 account for the specificity of CdiI-mediated immunity, we replaced the coding sequences for CdiA-CT536 and CdiI536 in UPEC 536 with the corresponding region from EC93. The resulting strain produces a chimeric CdiA protein, in which the C-terminal 223 residues of CdiAEC93 are fused to the N-terminal 3020 residues of CdiA536. UPEC 536 producing this chimeric CdiA inhibited target cells expressing CdiI536 but not cells expressing CdiIEC93, whereas the converse was true for wild-type UPEC 536 (Fig. 1c). These results show that CdiA-CTEC93 is functional when grafted onto the CdiA molecule from UPEC 536, and that the CdiA-CT sequence is important for specificity of immunity.

The observation that CdiI-mediated immunity is specific to the CdiA-CT suggests that growth inhibitory activity is also contained within the CdiA-CT. Expression of the C-terminal 268 residues of CdiAEC93 inside E. coli cells inhibited growth, and this inhibition was blocked by co-production of CdiIEC93, but not CdiI536 (Fig. 2a). Because the CdiA-CT lacks a secretion signal sequence, it is likely that this CdiA-CT-mediated growth inhibition and CdiI-mediated immunity occur within the cytoplasm. The minimal active region of CdiA-CTEC93 was determined by deletion analysis. Removal of up to 25 residues from the N-terminus of the 268 residue CdiA-CTEC93 construct did not significantly affect growth inhibitory activity (Fig. 2b). Removal of 45 residues from the N-terminus, which includes the conserved VENN motif, yielded a polypeptide with ∼10-fold greater inhibitory activity. Deletion of an additional 13 residues from the N-terminus completely abrogated activity (Fig. 2b). Deletion of as few as 12 residues from the C-terminus of the CdiA-CTEC93 construct also abolished inhibitory activity. Thus, the growth inhibitory activity resides within the C-terminal 223 amino acid residues of CdiAEC93.

We next searched for additional cdiBAI loci in other E. coli strains. Although several cdiBAI gene clusters are present in partially assembled E. coli genome sequences (Supplementary Table 1), we limited our analysis to the 33 fully assembled E. coli genomes currently available. Two E. coli isolates, UTI89 and CFT073, encode two-partner secretion systems related to EC93 CdiB/CdiA. The UTI89 CDI module is identical to that of UPEC 536 and is located within the same pathogenicity island (PAI II536) in both strains (Supplementary Fig. 2a)7. In contrast, the CFT073 CDI locus resides in an unrelated pathogenicity island (PAI-CFT073-aspV) 5, and its predicted CdiA-CT and CdiI sequences show no similarity to the UPEC 536 or EC93 systems (Supplementary Figs. 2b, 2c, 3b). The EC93 CdiA-CT is unrelated to the CdiA-CTs identified in other fully sequenced E. coli strains; however, it is 42% identical to the CdiA-CT from another species, Edwardsiella tarda EIB202. This, together with the sporadic occurrence of cdi loci in E. coli strains and their association with genomic islands, suggests that these genes may be transferred horizontally.

Bioinformatic analyses showed that CDI systems are widespread amongst other Gram-negative bacteria, with representatives identified in a variety of α-, β-, and γ-proteobacteria (Supplementary Table 1 and Supplementary Fig. 3). Although widely distributed, only certain strains of any given species contain cdiBAI homologues. Some bacterial isolates encode multiple CDI modules, for example Bartonella grahamii as4aup and Photorhabdus luminescens subsp. laumondii contain four and five CDI modules, respectively (Supplementary Fig. 3b). Based on their similarity to CdiAEC93 each putative cdiA homologue is likely to encode a TpsA family member with an N-terminal hemagglutination activity domain (Pfam PF05860, also called a TPS domain) and hemagglutinin repeats that are predicted to form a β-helical structure. Additionally, most cdiA homologs encode the VENN peptide motif, which is part of the DUF638 domain of unknown function (Pfam PF04829) (Fig. 1b and Supplementary Fig. 3a). In general, significant variability between CdiA-CT/CdiI protein sequences was observed between different species and between different CdiA-CTs encoded within a single strain. There are instances where an extended genomic region, including the CDI module, is conserved between different strains of the same species (as in E. coli UPEC 536 and UTI89, and most Y. pestis strains); in such cases the entire CdiA protein and CdiI are highly conserved. Burkholderia species also appear to encode CdiB/CdiA two-partner secretions systems, but these loci have a different gene organization (cdiAIB rather than cdiBAI), and the putative CdiA proteins lack the DUF638 domain. Instead of the VENN motif, the Burkholderia cdiA homologues encode an NxxLYN motif that precedes variable C-terminal domains (Supplementary Fig. 3c). In all instances, the Burkholderia cdiA genes are followed by short ORFs, which may be analogous to the cdiI genes in E. coli.

To determine whether the in silico-identified cdi loci encode functional CDI systems, we replaced the CdiA-CT and CdiI coding regions of UPEC 536 with the corresponding sequences from Yersinia pestis CO92 and the region 2 CDI module from Dickeya dadantii 3937 (Supplementary Table 1). UPEC 536 producing chimeric CdiAs inhibited target cells expressing heterologous CdiI proteins but not cells that express cognate CdiI (Fig. 1c), strongly suggesting that Y. pestis CO92 and D. dadantii 3937 encode CDI systems with allele-specific immunity proteins. These data also indicate that the N-terminal 3020 residues of CdiA536 are capable of delivering functional CdiA-CT domains from Y. pestis CO92 and D. dadantii 3937 into target cells.

How do CdiI immunity proteins protect against cognate CdiA-CT? We hypothesized that CdiI prevents CDI-mediated autoinhibition by specifically binding to the C-terminus of cognate CdiA. To test this hypothesis, we examined the interaction between CdiA-CT and hexahistidine-tagged CdiI (CdiI-His6) proteins using Ni2+-affinity pull-down experiments. Because the CdiA-CTs used in these experiments lack His6- epitope tags, their retention on Ni2+-nitrilotriacetic acid (Ni2+-NTA) resin is dependent upon binding to CdiI-His6. CdiA-CT536 was retained by the Ni2+-NTA resin when pre-incubated with CdiI536-His6, but not with non-cognate CdiI3937-2-His6 (Fig. 3a). Reciprocally, CdiA-CT3937-2 bound to the resin only in the presence of cognate CdiI3937-2-His6 (Fig. 3a). These data indicate that CdiI proteins bind to CdiA-CT in vitro in an allele-specific manner. To determine if CdiA-CT and CdiI bind one another in vivo, we used a modified bacterial two-hybrid system (BACTH, Euromedex). In this system, proteins of interest are fused to the T18 and T25 domains of adenylate cyclase, and binding of the two fusion proteins results in the production of cAMP, which is monitored indirectly through the expression of a β-galactosidase reporter 8. Co-expression of homologous T25-CdiA-CT536 and CdiI 536-T18 fusions, or T25-CdiA-CT3937-2 and CdiI3937-2-T18 fusions yielded high β-galactosidase activities in both cases, over 300 units (Fig. 3b). Because CdiA-CTs are cytotoxic in the absence of cognate CdiI, we used green fluorescent protein (GFP) as a negative control to test binding specificity. Co-expression of T25-GFP and CdiI536-T18, or T25-GFP and CdiI3937-2 -T18 resulted in very low β-galactosidase activities (Fig. 3b). Taken together, these data demonstrate that CdiI immunity proteins bind to their cognate CdiA-CTs in vitro and in vivo.

What is the mechanism(s) by which CDI systems inhibit target cell growth? Most CdiA-CTs are not similar to known proteins or protein domains. However, we found that the C-terminal 132 residues of CdiA3937-2 from D. dadantii 3937 shares 35% identity with the pyocin S3 nuclease domain from Pseudomonas aeruginosa (Supplementary Fig. 4). Pyocin S3 is cytotoxic by virtue of its DNase activity 9, suggesting that CdiA3937-2 may also use nuclease activity to inhibit target cell growth. We confirmed that purified CdiA-CT3937-2 possesses a robust Mg2+-dependent DNase activity, capable of completely digesting linear and supercoiled plasmid DNA (Fig. 3c, and data not shown). We also examined the activity of CdiA-CT536, which does not share sequence homology with other known toxins or colicins, and found that it has tRNase activity (Fig. 3d). Purified CdiA-CT536 readily cleaved several E. coli tRNA species, but not ribosomal RNA or messenger RNA (Fig. 3d, and data not shown). For each CdiA-CT, the addition of purified cognate CdiI blocked nuclease activity, whereas addition of heterologous CdiI had no effect on activity (Fig. 3c, 3d). These results suggest that CDI systems use more than one mechanism to inhibit cell growth. If these DNase and tRNase activities are responsible for growth inhibition, then the CdiA-CTs must be translocated into the target cell cytoplasm (Supplementary Fig. 1). According to this model, CdiI proteins confer immunity to CDI by binding to cognate CdiA-CTs and blocking their enzymatic activities. Based on the diversity of CdiA-CT sequences (Fig. 1b, Supplementary Fig. 3), it seems likely that additional growth inhibitory mechanisms will be identified for other CDI systems.

It is not known when or where CDI systems are deployed in the environment, nor what precise biological function(s) they provide. UPEC 536 does not express the cdiBAI gene cluster under standard laboratory growth conditions (unpublished data). However, EC93 expresses cdiBAI constitutively 2. To determine if the CDI system in EC93 provides a selective advantage, we deleted the CdiA-CT and CdiI coding sequences of EC93 and mixed the resulting mutant cells with wild-type EC93 at a one-to-one ratio in a growth competition experiment. After three hours of co-culture, EC93ΔcdiA-CTΔcdiI mutant bacteria were less than 1% of wild-type EC93 cells (competitive index <10-2, Fig. 4a). However, EC93ΔcdiA-CTΔcdiI cells expressing CdiIEC93 from a plasmid were able to compete equally with wild-type EC93, indicating that the original loss of fitness was due to the mutant's susceptibility to CDI (Fig. 4a). These results indicate that CDI systems may play a significant role in intraspecies competition between bacteria occupying the same ecological niche. Further support for this conclusion came from analysis of D. dadantii. Previous work has shown that disruption of a putative cdiI gene, designated virA, reduces the virulence of D. dadantii EC16 on plant hosts 10. Our results indicate that VirA binds and inactivates the C-terminal domain of HecA EC16, a CdiA homologue (Supplementary Table 1 and data not shown). These results suggest that D. dadantii may express cdi genes on plants. We used the fully sequenced D. dadantii 3937 strain, which contains two CDI regions (cdi3937-1 and cdi3937-2, see Supplementary Table 1), to test the hypothesis that CDI plays a role in intrastrain competition. Each of the cdiA-CT/cdiI regions were deleted individually, and the resulting mutants were competed against wild-type D. dadantii 3937 on chicory 11, 12. Although deletion of the cdi3937-2 region had no effect on competition, cells lacking cdi3937-1 were outcompeted by wild-type bacteria as evidenced by a competitive index of about 10-1 (Fig. 4b and data not shown). This competitive disadvantage was reversed by complementation with a chromosomal copy of the cognate cdiI3937-1 gene (Fig. 4b). Complementation was specific as non-cognate cdiIEC16 (virA) from D. dadantii EC16, had no effect on the competitive index (Fig. 4b). These results show that the region 1 CDI system in D. dadantii plays a role in growth competition on chicory. The role of the region 2 CDI system is unknown, but could function under different environmental conditions or target different bacterial species. Together, these results strongly indicate that CDI systems function in growth competition in the environment.

Methods Summary

The complete methods are presented in the Supplementary Information. Strains, plasmids, and oligonucleotides used in this study are shown in Supplementary Table 2. E. coli competition assays were carried out as previously described 2. D. dadantii competition assays on chicory were carried out as described in Supplementary Methods. EC93 and D. dadantii 3937 cdiA-CT-cdiI deletions and CdiA chimeras were constructed using allelic exchange as previously described 13. For chimera construction, the 3′ end of cdiA and all of cdiI from UPEC 536 ΔkpsK15 ΔaraCBA specRExBAD-cdiBAI (DL5646) were replaced with cdiA-CT (sequence immediately following VENNX) and cdiI from E. coli EC93, Y. pestis CO92 (accession number Q7CGD9), or D. dadantii 3937 region 2 (see Supplementary Methods). The ΔkpsK15 capsule mutation was used to increase the efficacy of CDI, based on our previous results showing that capsule production blocks CDI 3. Immunity plasmids were constructed by ligating PCR-amplified cdiI genes into plasmid pBR322 under tet promoter control (Fig. 1a, c). For D.dadantii 3937 the immunity plasmids were constructed by ligating PCR-amplified cdiI genes into the miniTn7 delivery plasmid pUC18R6KT-miniTn7T under tet promoter control (see Supplementary methods). Deletion mapping of E. coli EC93 cdiA-CT (Fig. 2b) was carried out by cloning specific sequences amplified by PCR into plasmid pLAC1114 under lac promoter control. All plasmids were propagated in EPI100 acrB mutant strain DL5154 to mitigate toxic effects. In vivo interactions between CdiA-CT and CdiI were determined using a modified BACTH bacterial two-hybrid system (Euromedex) 8. β-galactosidase14 and fluorescence3 analyses were carried out as previously described. In vitro affinity pull-downs with His6-tagged CdiI/CdiA-CT were carried out using Ni2+-NTA resin (Qiagen) (Fig. 3a). CdiA-CT was released by denaturation in buffer containing 6 M guanidine-HCl, and His6-tagged CdiI was released in native buffer supplemented with 250 mM imidazole. CdiA-CT activities were analyzed as described in supplementary methods.


FN4Author Contributions: D.A.L., S.K.A., P.A.C., and C.S.H. designed research. The manuscript was prepared by D.A.L., C.S.H., P.A.C., and S.J.P., and S.K.A.. S.J.P. and B.R.B. carried out bioinformatic analyses. B.R.B., A.M.J., and P.A.C. obtained initial evidence for the toxic nature of CdiA-CT, variability of CdiA-CTs and CdiIs, and binding between CdiA-CTs and cognate CdiIs in studies of Burkholderia pseudomallei cdi genes. S.K.A. cloned and performed competition assays and growth curves involving cdiBAI, cdiA-CT, and cdiA chimeras and deletions. B.A.B. and J.S.W. conducted the deletion mapping study with assistance from S.K.A. B.A.B. and S.K.A. conducted the bacterial two-hybrid study. C. T. R. constructed D.dadantii cdi mutants and plasmids and performed growth competition assays on chicory. E.J.D. cloned, purified protein, and performed the in vitro protein interaction and CdiA-CT activity studies.

FN5Author Information. Reprints and permissions information is available at The authors declare no competing financial interests. Readers are welcome to comment on the online version of this article at

We thank A. Charkowski (University of Wisconsin), A. Collmer (Cornell University), J. Roth (University of California, Davis) and H. Schweizer (Colorado State University) for plasmids, bacterial strains, and helpful discussions, W. Lathem (Northwestern University, Chicago, IL) for Y. pestis CO92 DNA, and R. Christoffersen (University of California, Santa Barbara) for helpful advice on plant experiments. This work was supported by National Science Foundation grant 0642052 (D.A.L.), a Tri-Counties Blood Bank Postdoctoral Fellowship (S.K.A.), and National Institutes of Health grants GM078634 (C.S.H.) and U54AI065359 from the National Institute of Allergy and Infectious Diseases (D.A.L., P.A.C., and C.S.H.). The content is the sole responsibility of the authors and does not necessarily represent the official views of the National Institute of Allergy and Infectious Diseases or the National Institutes of Health. This project made use of preliminary sequences from the Dickeya dadantii 3937 genome project supported by the Initiative for Future Agriculture and Food Systems grant no. 2001-52100-11316 from the USDA Cooperative State Research, Education, and Extension Service.

1. Hibbing ME,Fuqua C,Parsek MR,Peterson SB. Bacterial competition: surviving and thriving in the microbial jungleNat Rev Microbiol 8:15–25. [pmid: 19946288]
2. Aoki SK,et al. Contact-dependent inhibition of growth in Escherichia coliScience 309:1245–8.2005; [pmid: 16109881]
3. Aoki SK,et al. Contact-dependent growth inhibition requires the essential outer membrane protein BamA (YaeT) as the receptor and the inner membrane transport protein AcrBMol Microbiol 70:323–40.2008; [pmid: 18761695]
4. Aoki SK,Webb JS,Braaten BA,Low DA. Contact-dependent growth inhibition causes reversible metabolic downregulation in Escherichia coliJ Bacteriol 191:1777–86.2009; [pmid: 19124575]
5. Luo C,Hu GQ,Zhu H. Genome reannotation of Escherichia coli CFT073 with new insights into virulenceBMC Genomics 10:552.2009; [pmid: 19930606]
6. Pagni M,Jongeneel CV. Making sense of score statistics for sequence alignmentsBrief Bioinform 2:51–67.2001; [pmid: 11465063]
7. Dobrindt U,et al. Genetic structure and distribution of four pathogenicity islands (PAI I(536) to PAI IV(536)) of uropathogenic Escherichia coli strain 536Infect Immun 70:6365–72.2002; [pmid: 12379716]
8. Karimova G,Pidoux J,Ullmann A,Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathwayProc Natl Acad Sci U S A 95:5752–6.1998; [pmid: 9576956]
9. Duport C,Baysse C,Michel-Briand Y. Molecular characterization of pyocin S3, a novel S-type pyocin from Pseudomonas aeruginosaJ Biol Chem 270:8920–7.1995; [pmid: 7721800]
10. Rojas CM,et al. The Erwinia chrysanthemi EC16 hrp/hrc gene cluster encodes an active Hrp type III secretion system that is flanked by virulence genes functionally unrelated to the Hrp systemMol Plant Microbe Interact 17:644–53.2004; [pmid: 15195947]
11. Ham JH,et al. Analysis of Erwinia chrysanthemi EC16 pelE∷uidA, pelL∷uidA, and hrpN∷uidA mutants reveals strain-specific atypical regulation of the Hrp type III secretion systemMol Plant Microbe Interact 17:184–94.2004; [pmid: 14964532]
12. Yap MN,Rojas CM,Yang CH,Charkowski AO. Harpin mediates cell aggregation in Erwinia chrysanthemi 3937J Bacteriol 188:2280–4.2006; [pmid: 16513758]
13. Edwards RA,Keller LH,Schifferli DM. Improved allelic exchange vectors and their use to analyze 987P fimbria gene expressionGene 207:149–57.1998; [pmid: 9511756]
14. Braaten BA,Nou X,Kaltenbach LS,Low DA. Methylation patterns in pap regulatory DNA control pyelonephritis-associated pili phase variation in E. coliCell 76:577–88.1994; [pmid: 7906204]


[Figure ID: F1]
Figure 1  Analysis of CdiA chimeras

a, Target cells expressing CdiIEC93 or CdiI536 were co-cultured with inhibitor cells expressing cdi genes from either E. coli EC93 or UPEC 536. After 3 h, the number of viable target cells was determined as colony forming units (CFU) per ml (mean ± s.d., n = 4 experiments). b, The C-terminal ∼200 residues of the indicated CdiAs diverge following a conserved VENN peptide motif (shown by different colors). The CdiI proteins from each system are also highly variable. c, Target cells expressing CdiI from E. coli UPEC 536, E. coli EC93, Y. pestis CO92 (accession Q7CGD9), and D. dadantii 3937 (CDI module 2, see Supplementary Table 1) were protected from CDI mediated by chimeric CdiA536 proteins containing cognate, but not heterologous, CdiA-CT (mean ± s.d., n = 2 experiments).

[Figure ID: F2]
Figure 2  CdiA-CT contains growth inhibitory activity

a, Growth of E. coli cells expressing CdiA-CTEC93 from plasmid pDAL778. Co-expression of cognate CdiIEC93, but not heterologous CdiI536, protected cells from growth inhibition. The mean ± s.d. is shown, n = 2 experiments. b, The 268 residue CdiA-CTEC93 peptide is depicted along with various truncation constructs indicating the number of residues deleted. Each CdiA-CT construct was tested for growth inhibitory activity when expressed in E. coli cells (“++” indicates that growth was blocked immediately after CdiA-CTEC93 induction, “+” indicates that growth was blocked after a 2 - 3 hr delay, and “-” indicates no growth inhibition).

[Figure ID: F3]
Figure 3  CdiI immunity protein binds specifically to cognate CdiA-CT and blocks activity

a, Purified CdiA-CT and CdiI-His6 proteins from UPEC 536 and D. dadantii 3937 (CDI module 2, 3937-2) were mixed in vitro with Ni2+-NTA resin. Aliquots of resin bound and unbound fractions were analyzed by SDS-PAGE and Coomassie blue staining. b, A bacterial two-hybrid system (BACTH) based on adenylate cyclase activity was used to monitor CdiA-CT/CdiI binding in vivo. A β-galactosidase reporter was used to measure adenylate cyclase activity. Expression of two-hybrid T25-cdiA-CT/cdiI-T18 fusion constructs resulted in significant β-galactosidase activity. T25-gfpmut3/cdiI-T18 fusions were used to control for background β-galactosidase activity. Fluorescence microscopy of cells expressing each D. dadantii 3937 construct confirms GFP expression of the control. P values were obtained using an unpaired, two-tailed t-test (mean ± s.d., n = 2 experiments). c, Purified CdiA-CT3937-2 was incubated with linear pUC19 DNA in the presence and absence of cognate and heterologous CdiI. Reactions were analyzed by native agarose gel electrophoresis and ethidium bromide staining. d, Purified CdiA-CT536 was incubated with an S100 cell extract (100,000 x g supernatant), in the presence and absence of cognate and heterologous CdiI. Reactions were analyzed by denaturing gel electrophoresis. Top panel, ethidium bromide (EtBr) staining for total tRNA (arrow indicates tRNA; asterisk indicates degradation products); lower panels, Northern blot analyses of tRNAHis and tRNA1BAla. Arrows indicate full-length tRNAs.

[Figure ID: F4]
Figure 4  CDI systems function in intrastrain growth competition

a, StrR CDI+ EC93 cells were mixed 1:1 with rifR EC93 ΔcdiA-CTΔcdiI cells that either contained no plasmid (cdiI-), pBR322 plasmid (vector), or CdiIEC93-expressing plasmid (cdiIEC93). After 3 hr of co-culture, cells were plated onto LB medium, and rifR and strR CFU/ml were quantified to calculate the competitive index [(rifR CFU/strR CFU)3 hr / (rifR CFU/strR CFU) 0 hr]. The mean ± s.d. is shown (n = 2 experiments), P value = 0.002. b. GentR CDI+D. dadantii 3937 cells were mixed 100:1 with nalRD. dadantii 3937 ΔcdiA-CT3937-1 ΔcdiI3937-1 alone (cdiI-), or complemented with heterologous cdiI from D. dadantii EC16 (cdiIEC16), or cognate cdiI (cdiI3937-1). Cell mixtures were inoculated onto chicory leaves (see Supplementary methods), incubated for the indicated times, and viable counts were determined. The competitive index (CFU nalR /CFU gentR) was calculated as described for panel ‘a’ above at each time point. The mean ± s.d. is shown (n = 2 experiments). P value at 24 h = 0.00004. P values were obtained using an unpaired, two-tailed t-test.

Article Categories:
  • Article

Previous Document:  The trophic fingerprint of marine fisheries.
Next Document:  L1 retrotransposition in neurons is modulated by MeCP2.