| A whole blood lymphocyte proliferation assay in healthy Thais: comparison of heparinized blood and acid citrate dextrose blood. | |
| | |
MedLine Citation:
|
PMID: 21302396 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
The lymphocyte proliferation assay (LPA) is a technique to determine T-lymphocyte functions in vitro. The standard LPA using peripheral blood mononuclear cells (PBMC) separated from heparinized blood requires a large blood sample, time consuming and expensive. It is more useful if acid citrate dextrose (ACD) blood could be used not only for LPA but also for other purposes. To determine whether whole blood composing between heparinized blood and ACD blood could be substituted for standard LPA using PBMC. Heparinized and ACD blood of 35 healthy Thai blood donors were studied herein. PBMC separated by density gradient centrifugation and diluted heparinized and ACD blood were used to test and compare for lymphoproliferative responses to phytohemagglutinin (PHA), pokeweed mitogen (PWM), and tetanus toxoid. A stimulation index (SI) for each mitogen or antigen was calculated. All Thai blood donors demonstrated positive proliferative responses to PHA and PWM by using PBMC and whole blood culture assays from both heparinized and ACD blood. However, the difference in the frequency of positive proliferative responses to tetanus toxoid by using PBMC and whole blood culture assays was significant. Nevertheless, no significant difference in frequency of positive responses to tetanus toxoid between heparinized and ACD blood was observed. This results suggested that no significant difference between using heparinized and ACD blood in standard LPA using PBMC. However, the whole blood LPA for measuring mitogen induced lymphoproliferation could be substituted for standard LPA from heparinized andACD blood. Whole blood LPA is easy, rapid, and more cost effective than PBMC culture assay. Thus, it would be applicable in a clinical laboratory as well as in research setting. |
| | |
Authors:
|
Thippawan Chuenchitra; Suchitra Sukwit; Pittapun Chaitaveep; Sukunya Kuvanont; Patcharin Amlee; Kamolwan Songprasom; Sutchana Tabprasit; Mark De Souza; Surangrat Srisurapanon; Narongrid Sirisopana; Sorachai Nitayaphan |
Related Documents
:
|
17244646 - Microvascular endowment in the developing chicken embryo lung. 16413216 - Breath tests and airway gas exchange. 17428016 - Electrospray ionization/atmospheric pressure photoionization multimode source for low-f... 12485076 - Effect of poloidal density variation of neutral atoms on the tokamak edge. 2440686 - Lipid peroxidation is not a major factor involved in the edema formation in perfused lu... 15660076 - Hematogenous infection of the human temporomandibular joint. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of the Medical Association of Thailand = Chotmaihet thangphaet Volume: 93 Suppl 2 ISSN: 0125-2208 ISO Abbreviation: J Med Assoc Thai Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2011-02-08 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7507216 Medline TA: J Med Assoc Thai Country: Thailand |
Other Details:
|
Languages: eng Pagination: S21-6 Citation Subset: IM |
Affiliation:
|
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. ThippawanC@afrims.org |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Blepharoptosis repaired by frontalis-orbicularis oculi flap: a new technique.
Next Document: Prevalence of overweight and obesity among school children in suburb Thailand defined by the Interna...