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wbeT sequence typing and IS1004 profiling of Vibrio cholerae isolates.
MedLine Citation:
PMID:  22225492     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  To investigate the molecular basis for serotype variation in Vibrio cholerae O1 and the genetic relatedness among different serotypes isolated from 2004-2008 in Iran. Methods and Results:  Despite the presence of all three serotypes of V. cholerae O1 (Ogawa, Inaba, Hikojima) in Iran in the last decade, the Inaba strains have been the dominated serotype. Sequence analysis of wbeT determined only a single substitution of G for A at position 295 in all Inaba strains resulting in a replacement of serine to proline. No difference was found in the copy numbers and profile of IS1004 between the classical and El Tor V. cholerae O1 strains, supporting the clonality amongst the isolates obtained over 5 years in Iran. In addition, Southern blots of HpaII-digested chromosomal DNAs of our Ogawa and Inaba isolates showed the presence of an incomplete copy of IS1004 for all isolates. Conclusions:  IS1004 profiling can be a reliable method for analysis of clonal dissemination of V. cholerae. The results indicated that specific point mutation at a particular position within the wbeT of V. cholerae O1 strains in Iran may occur which, in turn, may result in serotype switching. Significance and Impact of the Study:  Understanding the molecular basis for serotype conversion of V. cholerae and their genetic relatedness could give insights for the incoming cholera epidemic prediction and control. © 2012 The Authors Letters in Applied Microbiology© 2012 The Society for Applied Microbiology.
Authors:
Afsoon Sharifnia; Bita Bakhshi; Mohammad R Pourshafie
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-7
Journal Detail:
Title:  Letters in applied microbiology     Volume:  -     ISSN:  1472-765X     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran Department of Bacteriology, Tarbiat Modares University, Tehran, Iran.
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