Document Detail


The ways of amniotic fluid sampling and its influence on lamellar body count.
MedLine Citation:
PMID:  21443154     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Even though artificial surfactant is now available, respiratory distress syndrome still remains a serious problem in neonatology. Prenatal analysis of the amniotic fluid can provide data giving insight into the fetal lung maturity, which enables planning of the further outcome of high-risk pregnancies. Surfactant prevents atelectasis by forming a layer rich in phospholipids between the air and liquid phase in alveoli thus leading to increased surface tension in them, which is a precondition for a good lung function after birth. Lamellar bodies are a form of stored surfactant, and their count in the amniotic fluid can be determined simply by a standard hematology analyzer. The method of determining lamellar body count has found an important place in prenatal diagnostics and is recommended as an initial method of a "cascade" procedure of testing fetal lung maturity. However, considering the importance of procedure of sample collection, storage and centrifugation, which can significantly affect the results obtained for the lung maturity, the amniotic fluid samples must be absolutely free of contamination with blood, meconium, mucus, bacteria and leucocytes.
Authors:
Jovana Visnjevac; Aleksandra Novakov-Mikić; Aleksandra Nikolić
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Medicinski pregled     Volume:  63     ISSN:  0025-8105     ISO Abbreviation:  Med. Pregl.     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2011-03-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985249R     Medline TA:  Med Pregl     Country:  Serbia    
Other Details:
Languages:  eng     Pagination:  483-6     Citation Subset:  IM    
Affiliation:
Gynecology Consulting Room Visnjevac Novi Sad. jvisnjevac@gmail.com
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