| von Willebrand factor and occlusive arterial thrombosis. A study in normal and von Willebrand's disease pigs with diet-induced hypercholesterolemia and atherosclerosis. | |
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MedLine Citation:
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PMID: 2344301 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The thrombotic response of atherosclerotic arteries to stenosis and injury was studied in 14 pigs, eight normal and six with von Willebrand's disease (vWD). Atherosclerosis was produced by feeding a 1% to 2% cholesterol diet for 24 weeks. Both groups of pigs developed severe hypercholesterolemia, greater than five times baseline values. Coronary atherosclerosis was detected in all vWD pigs and in all but one normal pig and was not significantly different between groups. At sacrifice under general anesthesia, a Goldblatt clamp (GC) was positioned around the left anterior descending coronary (LAD) and carotid arteries to produce a stenotic segment, which was pinch-injured with needle holders. A 20 MHz Doppler velocity crystal was placed distal to the GC to detect cyclic flow reductions or permanent cessation of flow velocity indicative of occlusive thrombosis. In the phenotypically normal pigs with diet-induced atherosclerosis, occlusive thrombosis was detected in seven of seven LAD and seven of seven carotid arteries. In atherosclerotic vWD pigs, occlusive thrombosis failed to form in six LAD and 10 carotid arteries (p less than 0.003, Wilcoxon rank sum test). Scanning electron micrographs demonstrated platelet-fibrin microthrombi in both groups of pigs; only phenotypically normal pigs had occlusive thrombi. Von Willebrand factor is essential for the development of occlusive thrombosis and appears to support the progression of a mixed microthrombus to an occlusive thrombus. |
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Authors:
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T C Nichols; D A Bellinger; D A Tate; R L Reddick; M S Read; G G Koch; K M Brinkhous; T R Griggs |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Arteriosclerosis (Dallas, Tex.) Volume: 10 ISSN: 0276-5047 ISO Abbreviation: Arteriosclerosis Publication Date: 1990 May-Jun |
Date Detail:
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Created Date: 1990-06-25 Completed Date: 1990-06-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8401388 Medline TA: Arteriosclerosis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 449-61 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arterial Occlusive Diseases / etiology* Arteries Arteriosclerosis / complications*, etiology, pathology Blood Flow Velocity Carotid Arteries / pathology Cholesterol / blood Constriction, Pathologic Coronary Vessels / pathology Diet, Atherogenic Hemodynamics Hypercholesterolemia / complications*, etiology Male Swine Thrombosis / etiology* von Willebrand Diseases / complications* von Willebrand Factor / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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FDR000259/FD/FDA HHS; HL01648/HL/NHLBI NIH HHS; HL26309/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/von Willebrand Factor; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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