| A voltage-gated chloride channel in ascidian embryos modulated by both the cell cycle clock and cell volume. | |
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MedLine Citation:
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PMID: 8576858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Eggs of the ascidian Boltenia villosa have an inwardly rectifying Cl- current whose amplitude varies by more than 10-fold during each cell cycle, the largest amplitude being at exit from M-phase. We examined whether this current was also sensitive to changes in cell volume. 2. Cell swelling, produced by direct inflation through a whole-cell recording pipette, greatly increased the amplitude of the Cl- current at all stages of the cell cycle in activated eggs. Swelling was much less effective in unfertilized eggs. 3. The increase in Cl- current amplitude continued for 10-20 min after an increase in diameter that was complete in 10 s, suggesting the involvement of a second messenger system in the response. 4. Treatment of unfertilized eggs with 6-dimethylaminopurine (DMAP), an inhibitor of cell cycle-dependent protein kinases, increased the amplitude of the Cl- current and its sensitivity to swelling to levels characteristic of fertilized eggs. 5. Osmotically produced swelling also increased Cl- current amplitude in unfertilized eggs. 6. We propose that dephosphorylation renders the Cl- channel functional, and that swelling or activation of the egg increases the sensitivity of the channel to dephosphorylation, perhaps by disrupting its links to the cytoskeleton. |
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Authors:
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M Villaz; J C Cinniger; W J Moody |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of physiology Volume: 488 ( Pt 3) ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 1995 Nov |
Date Detail:
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Created Date: 1996-03-12 Completed Date: 1996-03-12 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 689-99 Citation Subset: IM |
Affiliation:
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Department of Zoology, University of Washington, Seattle 98195, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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analogs & derivatives,
pharmacology Animals Artifacts Cell Cycle / physiology Cell Size / physiology Chloride Channels / physiology* Chlorides / metabolism Cyclic AMP / pharmacology Embryo, Nonmammalian / cytology, enzymology, ultrastructure Enzyme Inhibitors / pharmacology Ion Channel Gating / physiology Membrane Potentials / physiology Osmosis / physiology Protein Kinases / metabolism Urochordata / embryology* |
| Grant Support | |
ID/Acronym/Agency:
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GM07108/GM/NIGMS NIH HHS; HD17486/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chloride Channels; 0/Chlorides; 0/Enzyme Inhibitors; 60-92-4/Cyclic AMP; 73-24-5/Adenine; 938-55-6/N(6),N(6)-dimethyladenine; EC 2.7.-/Protein Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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