Document Detail

A voltage-gated chloride channel in ascidian embryos modulated by both the cell cycle clock and cell volume.
MedLine Citation:
PMID:  8576858     Owner:  NLM     Status:  MEDLINE    
1. Eggs of the ascidian Boltenia villosa have an inwardly rectifying Cl- current whose amplitude varies by more than 10-fold during each cell cycle, the largest amplitude being at exit from M-phase. We examined whether this current was also sensitive to changes in cell volume. 2. Cell swelling, produced by direct inflation through a whole-cell recording pipette, greatly increased the amplitude of the Cl- current at all stages of the cell cycle in activated eggs. Swelling was much less effective in unfertilized eggs. 3. The increase in Cl- current amplitude continued for 10-20 min after an increase in diameter that was complete in 10 s, suggesting the involvement of a second messenger system in the response. 4. Treatment of unfertilized eggs with 6-dimethylaminopurine (DMAP), an inhibitor of cell cycle-dependent protein kinases, increased the amplitude of the Cl- current and its sensitivity to swelling to levels characteristic of fertilized eggs. 5. Osmotically produced swelling also increased Cl- current amplitude in unfertilized eggs. 6. We propose that dephosphorylation renders the Cl- channel functional, and that swelling or activation of the egg increases the sensitivity of the channel to dephosphorylation, perhaps by disrupting its links to the cytoskeleton.
M Villaz; J C Cinniger; W J Moody
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of physiology     Volume:  488 ( Pt 3)     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-03-12     Completed Date:  1996-03-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  689-99     Citation Subset:  IM    
Department of Zoology, University of Washington, Seattle 98195, USA.
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MeSH Terms
Adenine / analogs & derivatives,  pharmacology
Cell Cycle / physiology
Cell Size / physiology
Chloride Channels / physiology*
Chlorides / metabolism
Cyclic AMP / pharmacology
Embryo, Nonmammalian / cytology,  enzymology,  ultrastructure
Enzyme Inhibitors / pharmacology
Ion Channel Gating / physiology
Membrane Potentials / physiology
Osmosis / physiology
Protein Kinases / metabolism
Urochordata / embryology*
Grant Support
Reg. No./Substance:
0/Chloride Channels; 0/Chlorides; 0/Enzyme Inhibitors; 60-92-4/Cyclic AMP; 73-24-5/Adenine; 938-55-6/N(6),N(6)-dimethyladenine; EC 2.7.-/Protein Kinases

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