| The vitamin D receptor: a primitive steroid receptor related to thyroid hormone receptor. | |
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MedLine Citation:
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PMID: 2838696 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously reported the cloning and sequencing of both the chicken and human vitamin D3 receptor cDNAs. A comparison of their deduced amino acid sequence with that of the other classic steroid hormone receptors and the receptor for thyroid hormone indicates that there are two regions of conservation between these molecules. The first is a 70 amino acid, cysteine-rich sequence (C1), the second region (C2) is a 62 amino acid region located towards the carboxyl terminus of the proteins. In other systems the former has been identified as a region responsible for DNA binding activity, whereas the latter represents the NH2-terminal boundary of the hormone binding domain. We present here evidence utilizing eucaryotic expression of cDNA encoding the hVDR C1 domain, followed by a DNA cellulose chromatography assay, which confirms that the DNA binding activity resides in this region of the receptor for vitamin D3. Additionally, the vitamin D3 receptor contains a 60 amino acid portion at its carboxyl terminus (C3) which exhibits homology with the receptor for thyroid hormone. Conservation in this region of the molecule is found only between homologous or closely related receptors. This indicates a relationship between the vitamin D3 receptor and the receptor for thyroid hormone and may suggest that they evolved from a single primordial gene. |
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Authors:
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D P McDonnell; J W Pike; B W O'Malley |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of steroid biochemistry Volume: 30 ISSN: 0022-4731 ISO Abbreviation: J. Steroid Biochem. Publication Date: 1988 |
Date Detail:
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Created Date: 1988-08-11 Completed Date: 1988-08-11 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0260125 Medline TA: J Steroid Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 41-6 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Calcitriol / metabolism Chickens DNA / genetics Humans Molecular Sequence Data Receptors, Calcitriol Receptors, Steroid / genetics* Receptors, Thyroid Hormone / genetics* Sequence Homology, Nucleic Acid Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Calcitriol; 0/Receptors, Steroid; 0/Receptors, Thyroid Hormone; 32222-06-3/Calcitriol; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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