Document Detail


The vital function of Fe3O4@Au nanocomposites for hydrolase biosensor design and its application in detection of methyl parathion.
MedLine Citation:
PMID:  23280070     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe(3)O(4) core was synthesized using cysteamine (SH-NH(2)) as linker, and characterized by TEM, XPS, UV and electrochemistry. Then a hydrolase biosensor, based on self-assembly of methyl parathion hydrolase (MPH) on the Fe(3)O(4)@Au nanocomposite, was developed for sensitive and selective detection of the organophosphorus pesticide (OP) methyl parathion. The magnetic nanocomposite provides an easy way to construct the enzyme biosensor by simply exerting an external magnetic field, and also provides a simple way to renew the electrode surface by removing the magnet. Unlike inhibition-based enzyme biosensors, the hydrolase is not poisoned by OPs and thus is reusable for continuous measurement. AuNPs not only provide a large surface area, high loading efficiency and fast electron transfer, but also stabilize the enzyme through electrostatic interactions. The MPH biosensor shows rapid response and high selectivity for detection of methyl parathion, with a linear range from 0.5 to 1000 ng mL(-1) and a detection limit of 0.1 ng mL(-1). It also shows acceptable reproducibility and stability. The simplicity and ease of operation of the proposed method has great potential for on-site detection of P-S containing pesticides and provides a promising strategy to construct a robust biosensor.
Authors:
Yuting Zhao; Weiying Zhang; Yuehe Lin; Dan Du
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-01-02
Journal Detail:
Title:  Nanoscale     Volume:  5     ISSN:  2040-3372     ISO Abbreviation:  Nanoscale     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-22     Completed Date:  2013-07-11     Revised Date:  2014-10-29    
Medline Journal Info:
Nlm Unique ID:  101525249     Medline TA:  Nanoscale     Country:  England    
Other Details:
Languages:  eng     Pagination:  1121-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / instrumentation*
Environmental Monitoring / instrumentation*
Equipment Design
Equipment Failure Analysis
Gold / chemistry*
Insecticides / analysis,  chemistry
Magnetite Nanoparticles / chemistry*
Magnetometry / instrumentation*
Methyl Parathion / analysis*,  chemistry
Phosphoric Monoester Hydrolases / chemistry*
Grant Support
ID/Acronym/Agency:
U01 NS058161/NS/NINDS NIH HHS; U01 NS058161-01/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Insecticides; 0/Magnetite Nanoparticles; 41BCL2O91D/Methyl Parathion; 7440-57-5/Gold; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.-/methyl parathion hydrolase, Pseudomonas

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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