Document Detail


The viral transactivator E1A regulates the mouse mammary tumor virus promoter in an isoform- and chromatin-specific manner.
MedLine Citation:
PMID:  11909860     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteins encoded by the adenovirus E1A gene regulate both cellular and viral genes to mediate effects on cell cycle, differentiation, and cell growth control. We have identified the mouse mammary tumor virus (MMTV) promoter as a target of E1A action and investigated the role nucleoprotein structure plays in its response to E1A. Both 12 and 13 S forms target the MMTV promoter when it has a disorganized and accessible chromatin configuration. However, whereas the 13 S form is stimulatory, the 12 S form is repressive. When the MMTV promoter adopts an organized and repressed chromatin structure, it is targeted only by the 13 S form, which stimulates it. Although evidence indicates that E1A interacts with the SWI/SNF remodeling complex, E1A had no effect on chromatin remodeling at the MMTV promoter in organized chromatin. Analysis of E1A mutants showed that stimulation of the MMTV promoter is mediated solely through conserved region 3 and does not require interaction with Rb, p300/CBP-associated factor, or CBP/p300. Imaging analysis showed that E1A colocalizes with MMTV sequences in vivo, suggesting that it functions directly at the promoter. These results indicate that E1A stimulates the MMTV promoter in a fashion independent of chromatin conformation and through a direct mechanism involving interaction with the basal transcription machinery.
Authors:
Edlyn Soeth; Denise B Thurber; Catharine L Smith
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-03-21
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  277     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-05-28     Completed Date:  2002-07-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19847-54     Citation Subset:  IM    
Affiliation:
Signal Transduction Group, Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenovirus E1A Proteins / metabolism*,  physiology*
Animals
Cell Line
Cell Nucleus / metabolism
Chromatin / metabolism
Fluorescent Antibody Technique, Indirect
Genetic Vectors
Luciferases / metabolism
Mammary Tumor Virus, Mouse / genetics*
Mice
Microscopy, Fluorescence
Mutation
Plasmids / metabolism
Promoter Regions, Genetic*
Protein Binding
Protein Isoforms
RNA / metabolism
Transcription, Genetic
Transfection
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Adenovirus E1A Proteins; 0/Chromatin; 0/Protein Isoforms; 63231-63-0/RNA; EC 1.13.12.-/Luciferases

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