Document Detail


The viability of fatty tissues within adipose aspirates after conventional liposuction: a comprehensive study.
MedLine Citation:
PMID:  15725835     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was conducted to evaluate the viability of fatty tissues within adipose aspirates after conventional liposuction and to examine their potential role as a source of donor material for possible future autogenous fat grafting. Samples of adipose aspirates (group I, n = 8) were obtained from adult female patients who underwent a conventional liposuction of the abdomen. Samples of fresh fatty tissues obtained from adult female patients who underwent an abdominoplasty (group II, n = 8) were cut into small pieces and served as a control. All samples were spun at 50 x g for 10 minutes; fatty tissues were then collected from the middle layer after centrifugation for the following studies: trypan blue vital staining for viable fatty cell counts, glycerol-3-phophatase dehydrogenase (G3PDH) assay for functional evaluation of fatty tissues, and routine pathology for histology of fatty tissues. There was no significant difference of viable fatty cell counts in group I compared with group II (2.57 +/- 0.56 versus 2.74 +/- 0.59 x 10/mL, P = 0.56). G3PGH assay showed a marked decrease of the enzyme activity in group I compared with group II (0.34 +/- 0.13 versus 0.76 +/- 0.13 micro/mL, P < 0.0001). Histologically, the normal structure of fatty tissues was found primarily in both groups. Our results indicate that although fatty tissues within adipose aspirates after conventional liposuction maintain normal structure with near the same number of viable fatty cells compared with fresh ones, they have a less-than-optimal level of cellular function and may not survive well after they are transplanted.
Authors:
Lee L Q Pu; Xiangdong Cui; Betsy F Fink; Michael L Cibull; Dayong Gao
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of plastic surgery     Volume:  54     ISSN:  0148-7043     ISO Abbreviation:  Ann Plast Surg     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-23     Completed Date:  2005-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7805336     Medline TA:  Ann Plast Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  288-92; discussion 292     Citation Subset:  IM    
Affiliation:
Division of Plastic Surgery, University of Kentucky, Lexington, KY 40536-0284, USA. lpu@uky.edu
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology,  physiology
Adipose Tissue / cytology,  physiology*,  transplantation*
Cell Count
Female
Humans
Lipectomy*
Tissue Survival / physiology*
Tissue and Organ Harvesting

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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