Document Detail


A vesicular stomatitis virus (cytomegalovirus) pseudotype and its use in neutralization tests.
MedLine Citation:
PMID:  227814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infection with vesicular stomatitis virus (VSV) of human diploid cells preinfected with the AD-169 strain of human cytomegalovirus (CMV) resulted in the formation of a VSV (CMV) pseudotype. Its formation was favored by increasing the bicarbonate content in doubly-infected cultures. The pseudotype was capable of infecting not only human but also rabbit cells. Pseudotype particles formed after infection with the tl 17 mutant of VSV, which carries a thermolabile lesion in its neutralization antigen, were more stable at 45 degrees than the original tl 17 virus. The pseudotype was used in the neutralization test with human sera. All sera positive for CMV antibody in the complement-fixation (CF) test were also reactive in the neutralization test. In addition, numerous sera negative for CMV antibody in the CF test neutralized the pseudotype.
Authors:
V Vonka; H Závadová; D Poláková
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Intervirology     Volume:  12     ISSN:  0300-5526     ISO Abbreviation:  Intervirology     Publication Date:  1979  
Date Detail:
Created Date:  1980-01-19     Completed Date:  1980-01-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0364265     Medline TA:  Intervirology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  32-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Viral / analysis
Child
Complement Fixation Tests
Cytomegalovirus / genetics*,  immunology
Humans
Mutation
Neutralization Tests*
Phenotype
Vesicular stomatitis Indiana virus / genetics*,  immunology
Viral Proteins / genetics*
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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