Document Detail

A vesicular stomatitis virus (cytomegalovirus) pseudotype and its use in neutralization tests.
MedLine Citation:
PMID:  227814     Owner:  NLM     Status:  MEDLINE    
Infection with vesicular stomatitis virus (VSV) of human diploid cells preinfected with the AD-169 strain of human cytomegalovirus (CMV) resulted in the formation of a VSV (CMV) pseudotype. Its formation was favored by increasing the bicarbonate content in doubly-infected cultures. The pseudotype was capable of infecting not only human but also rabbit cells. Pseudotype particles formed after infection with the tl 17 mutant of VSV, which carries a thermolabile lesion in its neutralization antigen, were more stable at 45 degrees than the original tl 17 virus. The pseudotype was used in the neutralization test with human sera. All sera positive for CMV antibody in the complement-fixation (CF) test were also reactive in the neutralization test. In addition, numerous sera negative for CMV antibody in the CF test neutralized the pseudotype.
V Vonka; H Závadová; D Poláková
Related Documents :
2512894 - Plaque quantitation and virus neutralization assays for human astroviruses.
8889804 - Real time observation of the binding of herpes simplex virus type 1 (hsv-1) to immobili...
2832344 - Irreproducibility of neutralization of herpes simplex virus under conditions where anti...
19481114 - Establishment of a neutralization test involving reporter gene-expressing virus-like pa...
11133384 - The hla a2/6802 supertype is associated with reduced risk of perinatal human immunodefi...
24031864 - Isolation and identification of feline calicivirus and feline herpesvirus in southern b...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Intervirology     Volume:  12     ISSN:  0300-5526     ISO Abbreviation:  Intervirology     Publication Date:  1979  
Date Detail:
Created Date:  1980-01-19     Completed Date:  1980-01-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0364265     Medline TA:  Intervirology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  32-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies, Viral / analysis
Complement Fixation Tests
Cytomegalovirus / genetics*,  immunology
Neutralization Tests*
Vesicular stomatitis Indiana virus / genetics*,  immunology
Viral Proteins / genetics*
Reg. No./Substance:
0/Antibodies, Viral; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Immunofluorescent anti-Junin virus antibodies in Argentine hemorrhagic fever.
Next Document:  The pathology of head and neck tumors: salivary glands, part 3.