Document Detail


DOTAP/UDCA vesicles: novel approach in oligonucleotide delivery.
MedLine Citation:
PMID:  17379164     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The relatively hydrophilic bile acid, ursodeoxycholic acid (UDCA), was used as an additive to DOTAP cationic liposomes to evaluate the effect on the cellular uptake of an oligonucleotide. Nuclear magnetic resonance studies were applied to estimate the relative amount of incorporated UDCA into the lipidic bilayers. DOTAP or DOTAP-UDCA vesicles (MixVes; DOTAP/UDCA molar ratios 1:0.25, 1:0.5, 1:1, and 1:2) formed complexes with 5'-fluorescein conjugated 29-mer phosphorothioate oligonucleotides (PS-ODNs) and studied using gel electrophoresis. In addition, the complexes were tested after transfection to assess the cellular uptake and the localization of the oligo in a HaCaT cell line by the use of cytofluorimetric and confocal microscopic analysis. DOTAP lipid formulated in the presence of a defined amount of UDCA forms more stable, flexible, and active MixVes. In particular, the MixVes at 1:0.25 and 1:0.5 molar ratios increase and modify the cellular uptake of PS-ODNs if compared with DOTAP liposomes 3 hours after the transfection studies. Moreover, the in vitro data suggest that these new formulations are not toxic.
Authors:
Barbara Ruozi; Renata Battini; Monica Montanari; Adele Mucci; Giovanni Tosi; Flavio Forni; Maria Angela Vandelli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nanomedicine : nanotechnology, biology, and medicine     Volume:  3     ISSN:  1549-9642     ISO Abbreviation:  -     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-23     Completed Date:  2007-05-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233142     Medline TA:  Nanomedicine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-13     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Modena, Italy.
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MeSH Terms
Descriptor/Qualifier:
Cell Death / drug effects
Drug Delivery Systems / methods*
Fatty Acids, Monounsaturated / pharmacology*
Humans
Liposomes / pharmacology*
Magnetic Resonance Spectroscopy
Microscopy, Atomic Force
Microscopy, Confocal
Oligonucleotides / administration & dosage*,  pharmacology*
Quaternary Ammonium Compounds / pharmacology*
Transfection
Ursodeoxycholic Acid / pharmacology*
Chemical
Reg. No./Substance:
0/Fatty Acids, Monounsaturated; 0/Liposomes; 0/Oligonucleotides; 0/Quaternary Ammonium Compounds; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 128-13-2/Ursodeoxycholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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