Document Detail


A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides.
MedLine Citation:
PMID:  11454341     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel mild procedure for the selective cleavage of ketosidic linkages is developed using ceric ammonium nitrate (CAN) in anhydrous N,N-dimethylformamide. Its application to lipopolysaccharides (LPS) is very significant because in the so far investigated LPS, the connection between the Lipid A region and the oligo(poly)saccharide part is always a keto-sugar. This procedure has been tested on LPS of Escherichia coli which contains Kdo as a linker between Lipid A and OPS and on Acinetobacter haemoliticus which contains D-glycero-D-talo-2-octulopyranosonic acid (Ko) as a linker and it performed efficiently in both cases.
Authors:
E Manzo; A Molinaro; E Bedini; C De Castro; M Parrilli
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  333     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-16     Completed Date:  2001-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  339-42     Citation Subset:  IM    
Affiliation:
Dipartimento di Chimica Organica e Biochimica, Università di Napoli 'Federico II', via Cintia, I-80126, Naples, Italy.
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MeSH Terms
Descriptor/Qualifier:
Acinetobacter / chemistry
Carbohydrate Sequence
Carbohydrates / chemistry*
Cerium
Dimethylformamide
Escherichia coli / chemistry
Lipid A / chemistry*
Lipopolysaccharides / chemistry*
Molecular Sequence Data
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Lipid A; 0/Lipopolysaccharides; 16774-21-3/ceric ammonium nitrate; 68-12-2/Dimethylformamide; 7440-45-1/Cerium

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