| A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides. | |
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MedLine Citation:
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PMID: 11454341 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel mild procedure for the selective cleavage of ketosidic linkages is developed using ceric ammonium nitrate (CAN) in anhydrous N,N-dimethylformamide. Its application to lipopolysaccharides (LPS) is very significant because in the so far investigated LPS, the connection between the Lipid A region and the oligo(poly)saccharide part is always a keto-sugar. This procedure has been tested on LPS of Escherichia coli which contains Kdo as a linker between Lipid A and OPS and on Acinetobacter haemoliticus which contains D-glycero-D-talo-2-octulopyranosonic acid (Ko) as a linker and it performed efficiently in both cases. |
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Authors:
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E Manzo; A Molinaro; E Bedini; C De Castro; M Parrilli |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Carbohydrate research Volume: 333 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-07-16 Completed Date: 2001-11-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 339-42 Citation Subset: IM |
Affiliation:
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Dipartimento di Chimica Organica e Biochimica, Università di Napoli 'Federico II', via Cintia, I-80126, Naples, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter
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chemistry Carbohydrate Sequence Carbohydrates / chemistry* Cerium Dimethylformamide Escherichia coli / chemistry Lipid A / chemistry* Lipopolysaccharides / chemistry* Molecular Sequence Data |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Lipid A; 0/Lipopolysaccharides; 16774-21-3/ceric ammonium nitrate; 68-12-2/Dimethylformamide; 7440-45-1/Cerium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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