Document Detail

A vertically transmitted HIV-1 gag-subtype variant detected in Taiwan.
MedLine Citation:
PMID:  1291763     Owner:  NLM     Status:  MEDLINE    
We used polymerase chain reaction (PCR) to amplify a segment, about 560 base pairs (bp), of HIV-1 gag DNA prepared from peripheral blood mononuclear cells of a seropositive Taiwanese pair of mother and infant. TM-1 and TC-1 clones of PCR-amplified DNA derived from the mother and infant, respectively, showed a 94.5% homology with each other. However, the TM-1 and TC-1 sequences exhibited lower degrees of homology, i.e. only 85.1% and 85.8%, respectively, with the corresponding gag segment of a North American HIV-1 subtype (HXB2), and 86.4% and 87.0%, respectively, with that of a Zairean HIV-1 subtype (Z2Z6). The divergence of TM-1 and TC-1 sequences from those of HXB2 and Z2Z6 is particularly prominent in the first (5' proximal) 200 bp of the cloned DNA segment, involving transitions more frequently than transversions. Two additional clones TM-2 and TC-2 derived from the mother and infant were sequenced for the first 200 bp. These four clones showed a high degree of homology (94.7-97.5%) among themselves, providing an evidence for transmission of the virus from the mother to the infant. These findings show the epidemiological value of PCR, and indicate the presence of a gag subtype of HIV-1 which is distinct from both the North American and Zairean subtypes according to the phylogenetic tree constructed.
H G Guo; K S Chang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of medical science & biology     Volume:  45     ISSN:  0021-5112     ISO Abbreviation:  Jpn. J. Med. Sci. Biol.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1993-03-26     Completed Date:  1993-03-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0243706     Medline TA:  Jpn J Med Sci Biol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  127-36     Citation Subset:  IM; J; X    
Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
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MeSH Terms
Base Sequence
Genes, gag*
HIV-1 / genetics*
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Homology, Nucleic Acid

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