Document Detail


A versatile valve-enabled microfluidic cell co-culture platform and demonstration of its applications to neurobiology and cancer biology.
MedLine Citation:
PMID:  21424383     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A versatile microfluidic platform allowing co-culture of multiple cell populations in close proximity with separate control of their microenvironments would be extremely valuable for many biological applications. Here, we report a simple and compact microfluidic platform that has these desirable features and allows for real-time, live-cell imaging of cell-cell interactions. Using a pneumatically/hydraulically controlled poly(dimethylsiloxane) (PDMS) valve barrier, distinct cell types can be cultured in side-by-side microfluidic chambers with their optimum culture media and treated separately without affecting the other cell population. The platform is capable of both two-dimensional and three-dimensional cell co-culture and through variations of the valve barrier design, the platform allows for cell-cell interactions through either direct cell contact or soluble factors alone. The platform has been used to perform dynamic imaging of synapse formation in hippocampal neurons by separate transfection of two groups of neurons with fluorescent pre- and post-synaptic protein markers. In addition, cross-migration of 4T1 tumor cells and endothelial cells has been studied under normoxic and hypoxic conditions, which revealed different migration patterns, suggesting the importance of the microenvironments in cell-cell interactions and biological activities.
Authors:
Yandong Gao; Devi Majumdar; Bojana Jovanovic; Candice Shaifer; P Charles Lin; Andries Zijlstra; Donna J Webb; Deyu Li
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biomedical microdevices     Volume:  13     ISSN:  1572-8781     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-02     Completed Date:  2011-08-15     Revised Date:  2014-01-07    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  539-48     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication
Cell Line, Tumor
Cell Movement
Coculture Techniques / instrumentation*
Dimethylpolysiloxanes / chemistry
Endothelial Cells / cytology*
Humans
Hydrodynamics
Mice
Microfluidic Analytical Techniques / instrumentation*
Neurons / cytology*
Pressure
Sepharose / chemistry
Grant Support
ID/Acronym/Agency:
GM092914/GM/NIGMS NIH HHS; MH071674/MH/NIMH NIH HHS; R01 CA143081/CA/NCI NIH HHS; R01 GM092914-01/GM/NIGMS NIH HHS; R01 GM092914-02/GM/NIGMS NIH HHS; R01 MH071674-05/MH/NIMH NIH HHS; S10RR025524/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Dimethylpolysiloxanes; 63148-62-9/baysilon; 9012-36-6/Sepharose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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