| A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants. | |
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MedLine Citation:
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PMID: 10940414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed an incubator housed, biaxial-tissue-loading device capable of applying axial deformations as small as 1 microm and sinusoidal rotations as small as 0.01 degrees. Axial resolution is 50 nm for applying sinewaves as low as 10 microm (or 1% based on a 1 mm thickness) or as large as 100 microm. Rotational resolution is 0.0005 degrees. The machine is small enough (30 cm high x 25 cm x 20 cm) to be placed in a standard incubator for long-term tissue culture loading studies. In metabolic studies described here, application of sinusoidal macroscopic shear deformation to articular cartilage explants resulted in a significant increase in the synthesis of proteoglycan and proteins (uptake of (35)S-sulfate and (3)H-proline) over controls held at the same static offset compression. |
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Authors:
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E H Frank; M Jin; A M Loening; M E Levenston; A J Grodzinsky |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of biomechanics Volume: 33 ISSN: 0021-9290 ISO Abbreviation: J Biomech Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-12-11 Completed Date: 2000-12-11 Revised Date: 2009-11-11 |
Medline Journal Info:
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Nlm Unique ID: 0157375 Medline TA: J Biomech Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1523-7 Citation Subset: IM |
Affiliation:
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Continuum Electromechanics Group, Center for Biomedical Engineering, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Room 38-377, Cambridge, MA 02139, USA. ehfrank@mit.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomechanics Biomedical Engineering / instrumentation Cartilage, Articular / metabolism, physiology* Cattle Culture Techniques Equipment Design Proline / metabolism Sulfates / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AR33236/AR/NIAMS NIH HHS; AR45779/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Sulfates; 147-85-3/Proline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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