Document Detail

A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants.
MedLine Citation:
PMID:  10940414     Owner:  NLM     Status:  MEDLINE    
We have developed an incubator housed, biaxial-tissue-loading device capable of applying axial deformations as small as 1 microm and sinusoidal rotations as small as 0.01 degrees. Axial resolution is 50 nm for applying sinewaves as low as 10 microm (or 1% based on a 1 mm thickness) or as large as 100 microm. Rotational resolution is 0.0005 degrees. The machine is small enough (30 cm high x 25 cm x 20 cm) to be placed in a standard incubator for long-term tissue culture loading studies. In metabolic studies described here, application of sinusoidal macroscopic shear deformation to articular cartilage explants resulted in a significant increase in the synthesis of proteoglycan and proteins (uptake of (35)S-sulfate and (3)H-proline) over controls held at the same static offset compression.
E H Frank; M Jin; A M Loening; M E Levenston; A J Grodzinsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomechanics     Volume:  33     ISSN:  0021-9290     ISO Abbreviation:  J Biomech     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-11     Completed Date:  2000-12-11     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0157375     Medline TA:  J Biomech     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1523-7     Citation Subset:  IM    
Continuum Electromechanics Group, Center for Biomedical Engineering, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Room 38-377, Cambridge, MA 02139, USA.
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MeSH Terms
Biomedical Engineering / instrumentation
Cartilage, Articular / metabolism,  physiology*
Culture Techniques
Equipment Design
Proline / metabolism
Sulfates / metabolism
Grant Support
Reg. No./Substance:
0/Sulfates; 147-85-3/Proline

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