Document Detail

A versatile fiber-optic coupled system for sensitive optical spectroscopy in strong ambient light.
MedLine Citation:
PMID:  19485494     Owner:  NLM     Status:  MEDLINE    
In this work we describe design and use of a fiber-optic based optical system for the spectroscopic studies on the samples under the presence of strong ambient light. The system is tested to monitor absorption, emission, and picosecond-resolved fluorescence transients simultaneously with a time interval of 500 ms for several hours on a biologically important sample (vitamin B2) under strong UV light. An efficient stray-light rejection ratio of the setup is achieved by the confocal geometry of the excitation and detection channels. It is demonstrated using this setup that even low optical signal from a liquid sample under strong UV-exposure for the picosecond-resolved fluorescence transient measurement can reliably be detected by ultrasensitive microchannel plate photomultiplier tube solid state detector. The kinetics of photodeterioration of vitamin B2 measured using our setup is consistent with that reported in the literature. Our present studies also justify the usage of tungsten light than the fluorescent light for the healthy preservation of food with vitamin B2.
Sudarson Sekhar Sinha; Pramod Kumar Verma; Abhinandan Makhal; Samir Kumar Pal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Review of scientific instruments     Volume:  80     ISSN:  1089-7623     ISO Abbreviation:  Rev Sci Instrum     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-06-02     Completed Date:  2009-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0405571     Medline TA:  Rev Sci Instrum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  053109     Citation Subset:  IM    
Department of Chemical, Biological and Macromolecular Sciences, Unit for Nano Science and Technology, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India.
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MeSH Terms
Optical Fibers*
Riboflavin / chemistry
Sensitivity and Specificity
Spectrum Analysis / instrumentation*
Time Factors
Reg. No./Substance:

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