Document Detail

A versatile alginate droplet generator applicable for microencapsulation of pancreatic islets.
MedLine Citation:
PMID:  10147997     Owner:  NLM     Status:  MEDLINE    
Alginate beads for immunoisolation of pancreatic islets by microencapsulation should be small, smooth, and spherical in order to ensure that around the islets a strong alginate-polylysine-alginate capsule will be formed with optimal biocompatibility and diffusion of nutrients and hormones. However, the preparation of small capsules around islets is difficult. Our newly designed air jet droplet generator allows for variations in the length and diameter of the alginate nozzle and the air jacket and is in this way adaptable to a required bead size. Alginate droplets are converted into rigid beads in a 100 mM CaCl 2 solution. Their size depends upon the diameter of the jacket, the air flow rate, and the outer diameter of the nozzle, whereas the production rate depends upon the pressure on the alginate, and on the diameter and the length of the nozzle. When the air flow or the alginate flow surpasses a certain rate, the droplets are fragmented. This study describes the mutual relationship of these variables and defines their optimal range for reproducible production of smooth and spherical beads for microencapsulation of islets at an acceptable production rate.
G H Wolters; W M Fritschy; D Gerrits; R van Schilfgaarde
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied biomaterials : an official journal of the Society for Biomaterials     Volume:  3     ISSN:  1045-4861     ISO Abbreviation:  J Appl Biomater     Publication Date:  1991  
Date Detail:
Created Date:  1993-04-22     Completed Date:  1993-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9011484     Medline TA:  J Appl Biomater     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  281-6     Citation Subset:  T    
Department of Surgery, University of Groningen, The Netherlands.
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MeSH Terms
Biocompatible Materials*
Drug Compounding / instrumentation*
Islets of Langerhans Transplantation / instrumentation*,  methods
Materials Testing
Reg. No./Substance:
0/Alginates; 0/Biocompatible Materials; 0/Capsules

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