| The variation of expression of CD4+ CD25+ Foxp3+ regulatory T cells in patients with Helicobacter pylori infection and eradication. | |
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MedLine Citation:
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PMID: 20698203 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: H. pylori persists for the virtual life of its host. Recent studies suggested that CD4+ CD25+ regulatory T cells may be involved in this process. However, the alteration of CD4+ CD25+ regulatory T cells after eradication of H. pylori remains a question. METHODOLOGY: By using biopsies from 45 H. pylori-positive patients and the ones after eradication of H. pylori and 35 H. pylori-negative adults, real-time PCR and general PCR were used to quantify the expression of Foxp3 mRNA. IHC was used to semi-quantify the number of CD4+ CD25+ T cells in gastric mucosa. RESULTS: We found that proportion of CD25+ T cell in CD4+ T cells accounted for 0.739% in H. pylori-negative individuals, while it was accounted for 5.012% in H. pylori-positive patients. After eradication of H. pylori, proportion of CD25+ T cell in CD4+ T cells declined (p < 0.01) and it accounted for 0.551%. The level of Foxp3 mRNA significantly decreased (p < 0.01) in gastric mucosa of patients after eradication of H. pylori. CONCLUSIONS: CD4+ CD25+ regulatory T cells decreased in gastric mucosa when patients received eradication of H. pylori. Eradication of H. pylori results in the significant descrease of Foxp3 mRNA in gastric mucosal, or using the drugs of anti-H. pylori induce the reduction of gastric mucosal Foxp3 mRNA expression, which is the a key regulatory gene for the development and function of CD4+ CD25+ regulatory T cells, thus contributing to the eradication of H. pylori. All the data offer new possibilities that Foxp3 gene may be the new target of immunization intervention strategies for eradication of H. pylori. |
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Authors:
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Tanzhou Chen; Ruifang Jin; Zhiming Huang; Wandong Hong; Zhoufeng Chen; Jinjin Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hepato-gastroenterology Volume: 57 ISSN: 0172-6390 ISO Abbreviation: Hepatogastroenterology Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-08-11 Completed Date: 2010-09-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8007849 Medline TA: Hepatogastroenterology Country: Greece |
Other Details:
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Languages: eng Pagination: 430-5 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology and Hepatology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult CD4-Positive T-Lymphocytes / physiology Female Forkhead Transcription Factors / analysis*, genetics Gastric Mucosa / immunology Helicobacter Infections / drug therapy, immunology* Helicobacter pylori* Humans Male Middle Aged RNA, Messenger / analysis T-Lymphocytes, Regulatory / physiology* |
| Chemical | |
Reg. No./Substance:
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0/FOXP3 protein, human; 0/Forkhead Transcription Factors; 0/RNA, Messenger |
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